1. Recombinant Proteins
  2. Cytokines and Growth Factors Immune Checkpoint Proteins CD Antigens
  3. TNF Superfamily Stimulatory immune checkpoint molecules T Cell CD Proteins Macrophage CD Proteins
  4. TNF Superfamily Ligands CD40 Ligand/CD154
  5. CD40 Ligand
  6. CD40L/CD154/TRAP Protein, Human (HEK 293, hFC-Flag)

CD40L/CD154/TRAP Protein, Human (HEK 293, hFC-Flag)

Cat. No.: HY-P72028
Handling Instructions
Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized CD40L at 2 μg/mL can bind Anti- CD40L Rabbit Monoclonal Antibody, the EC50 is 2.353-3.232 ng/mL.Measured by its binding ability in a functional ELISA. Immobilized CD40 at 2 μg/mL can bind
Species

Human

Source

HEK293

Tag

N-hFc;N-Flag

Accession

P29965 (M13-L261)

Gene ID
Synonyms
CD40-L; T-cell antigen Gp39; NF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD154; sCD40L;
AA Sequence

MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

Molecular Weight

Approximately 44.5 kDa

Purity

Greater than 93% as determined by SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in sterile distilled water.

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation
  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CD40L/CD154/TRAP Protein, Human (HEK 293, hFC-Flag)
Cat. No.:
HY-P72028
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