CD52 Protein, Rat (HEK293, Fc)

Customer Review

Based on 1 Customer Validation

CD52 protein's multifaceted role includes carrying and orienting carbohydrates, potentially involved in molecular transport and arrangement. Its dual nature suggests versatility in molecular processes, raising questions about its specific contributions. Further exploration into CD52's precise mechanisms and functions, as well as its more specific role, could provide insights into its broader significance. CD52 Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD52 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

CD52 protein's multifaceted role includes carrying and orienting carbohydrates, potentially involved in molecular transport and arrangement. Its dual nature suggests versatility in molecular processes, raising questions about its specific contributions. Further exploration into CD52's precise mechanisms and functions, as well as its more specific role, could provide insights into its broader significance. CD52 Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD52 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

The CD52 protein appears to have a multifaceted role, with implications in both carrying and orienting carbohydrates, suggesting a potential involvement in molecular transport and arrangement. Beyond its general function, CD52 may also have a more specific role, the details of which remain to be elucidated. The dual nature of CD52's potential roles hints at its versatility in molecular processes and raises intriguing questions about its specific contributions in cellular and molecular contexts. Further exploration into the precise mechanisms and functions associated with CD52's carbohydrate-related activities, as well as the identification of its more specific role, could provide valuable insights into its broader functional significance.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Rat is present at 10 μg/mL can bind CD52 antibody. The ED50 for this effect is 4.298 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant Rat CD52 is present at 10 μg/mL can bind CD52 antibody. The ED50 for this effect is 4.298 μg/mL.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-hFc
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • CD52 (G24-A69)
      Accession # Q63064
    • hFc
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    CD52; Cambridge Pathology 1 Antigen; Prev. CDW52; CDW52 Antigen (CAMPATH-1 Antigen); HE5; CD52 Antigen; CAMPATH-1 Antigen; CD52 Protein; EDDM5; HEL-S-171mP; Human Epididymis-Specific Protein 5; CDw52; CD52 Antigen (CAMPATH-1 Antigen); He5; Epididymal Secr

  • AA Sequence

    GQNSTAVTTPANKAATTAAATTKAAATTATKTTTAVRKTPGKPPKA

  • Molecular Weight

    Approximately 53 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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