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  2. CD Antigens Complement System
  3. Platelet CD Proteins Erythrocyte CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins Complement Regulatory Proteins
  4. DAF Protein/CD55 Decay Accelerating Factor (DAF)
  5. CD55/DAF Protein, Rat (HEK293, Fc)

CD55/DAF Protein, Rat (HEK293, Fc)

Cat. No.: HY-P76241
COA Handling Instructions

The CD55/DAF protein lacks conserved residues critical for feature annotation propagation and exhibits unique qualities that have prompted exploration of its structural and functional implications. This unique property suggests underlying nuances in molecular interactions and regulatory mechanisms. CD55/DAF Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD55/DAF protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD55/DAF Protein, Rat (HEK293, Fc) is 371 a.a., with molecular weight of 75-95 KDa.

For research use only. We do not sell to patients.

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Description

The CD55/DAF protein lacks conserved residues critical for feature annotation propagation and exhibits unique qualities that have prompted exploration of its structural and functional implications. This unique property suggests underlying nuances in molecular interactions and regulatory mechanisms. CD55/DAF Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD55/DAF protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD55/DAF Protein, Rat (HEK293, Fc) is 371 a.a., with molecular weight of 75-95 KDa.

Background

CD55, also known as Decay Accelerating Factor (DAF), exhibits a unique characteristic as it lacks conserved residue(s) essential for the propagation of feature annotation. This distinctive quality prompts further exploration into the structural and functional implications of CD55, emphasizing potential nuances in its molecular interactions and regulatory mechanisms. The absence of these conserved residues underscores the particularities of CD55 and suggests intriguing avenues for research to unravel its specific roles in immune regulation and complement system modulation.

Biological Activity

Immobilized Rat CD55 at 2 μg/mL (100 μL/well) can bind CD55 antibody. The ED50 for this effect is 1.221 μg/mL.

Species

Rat

Source

HEK293

Tag

C-hFc

Accession

Q9Z0M0 (M1-S371)

Gene ID

64036  [NCBI]

Molecular Construction
N-term
DAF (M1-S371)
Accession # Q9Z0M0
hFc
C-term
Synonyms
Complement Decay-Accelerating factor; CD55; CR; DAF
AA Sequence

DCGPPPDIPNATPNSGKHTKFAHQSQVTYSCNKGFKQIPDKPNTVVCLENDQWSNYETFCNKTCSAPLRLNYASLKKEYRNMNFFPIGTTVEFECRPGFQKVTSRTGKSTCLEELVWSPVVEFCKKRACPNPRELKNGHINIPTDILFGSEIIFSCDKGYKLVGVTSTFCSITGNAVDWDDVFPVCTTIFCPDPPKINNGRIREESDSYAYRQSVTYSCDQGFILVGNSSVHCTLNGEVGEWSSAPPKCIERHKVPTKTPPEVDIPSTMPHESTLINFPSTRAPLSHKPTTVKVPGTRVKPTSHKPTEVKVPATQHVPVSKTTVRHPTRTSKDRGES

Molecular Weight

Approximately 75-95 kDa due to the glycosylation.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD55/DAF Protein, Rat (HEK293, Fc)
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HY-P76241
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