CD55/DAF Protein, Rat (HEK293, Fc)
Based on 1 Customer Validation
The CD55/DAF protein lacks conserved residues critical for feature annotation propagation and exhibits unique qualities that have prompted exploration of its structural and functional implications. This unique property suggests underlying nuances in molecular interactions and regulatory mechanisms. CD55/DAF Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD55/DAF protein, expressed by HEK293 , with C-hFc labeled tag.
- Species: Rat
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The CD55/DAF protein lacks conserved residues critical for feature annotation propagation and exhibits unique qualities that have prompted exploration of its structural and functional implications. This unique property suggests underlying nuances in molecular interactions and regulatory mechanisms. CD55/DAF Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD55/DAF protein, expressed by HEK293 , with C-hFc labeled tag.
Background
CD55, also known as Decay Accelerating Factor (DAF), exhibits a unique characteristic as it lacks conserved residue(s) essential for the propagation of feature annotation. This distinctive quality prompts further exploration into the structural and functional implications of CD55, emphasizing potential nuances in its molecular interactions and regulatory mechanisms. The absence of these conserved residues underscores the particularities of CD55 and suggests intriguing avenues for research to unravel its specific roles in immune regulation and complement system modulation.
Verified Bioactivity
Immobilized Rat CD55 at 2 μg/mL (100 μL/well) can bind CD55 antibody. The ED50 for this effect is 1.221 μg/mL.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
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Bioactivity - ELISA
Bioactivity - ELISA
Technical Parameters
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Species Rat
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Source HEK293
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Tag C-hFc
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Accession
Q9Z0M0 (D35-S371)
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Molecular Construction
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N-term
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DAF (D35-S371)
Accession # Q9Z0M0 -
hFc
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C-term
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Protein Length
Partial
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Synonyms
CD55; CD55 Molecule, Decay Accelerating Factor For Complement (Cromer Blood Group); Prev. DAF; Rh Blood Group D Antigen; CROM; CD55 Antigen; CR; CD55 Antigen, Decay Accelerating Factor For Complement (Cromer Blood Group), Isoform CRA_d; Complement Decay-A
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AA Sequence
DCGPPPDIPNATPNSGKHTKFAHQSQVTYSCNKGFKQIPDKPNTVVCLENDQWSNYETFCNKTCSAPLRLNYASLKKEYRNMNFFPIGTTVEFECRPGFQKVTSRTGKSTCLEELVWSPVVEFCKKRACPNPRELKNGHINIPTDILFGSEIIFSCDKGYKLVGVTSTFCSITGNAVDWDDVFPVCTTIFCPDPPKINNGRIREESDSYAYRQSVTYSCDQGFILVGNSSVHCTLNGEVGEWSSAPPKCIERHKVPTKTPPEVDIPSTMPHESTLINFPSTRAPLSHKPTTVKVPGTRVKPTSHKPTEVKVPATQHVPVSKTTVRHPTRTSKDRGES
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Molecular Weight
Approximately 91 kDa & 54 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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