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  5. CD99 Protein, Rat (HEK293, Fc)

CD99 protein is a member of the CD99 family and plays a key role in several important cellular processes such as immune response, cell adhesion and migration. It exerts a major influence on lymphocyte trafficking and is involved in the regulation of immune cell interactions. CD99 Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD99 protein, expressed by HEK293 , with C-hFc labeled tag.

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제품 설명

CD99 protein is a member of the CD99 family and plays a key role in several important cellular processes such as immune response, cell adhesion and migration. It exerts a major influence on lymphocyte trafficking and is involved in the regulation of immune cell interactions. CD99 Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD99 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

CD99 Protein, a member of the CD99 family, is a key player in several important cellular processes, including immune responses, cell adhesion, and migration. It exerts significant influence on lymphocyte trafficking and is involved in the regulation of immune cell interactions. CD99 Protein is known to be expressed on various immune cells, where it contributes to the formation of immunological synapses and facilitates the communication between different immune cells. Additionally, CD99 Protein is implicated in cell adhesion and migration processes, playing a crucial role in the transmigration of leukocytes across endothelial cell barriers. Through its multifaceted functions, CD99 Protein is essential for the proper functioning of the immune system and the coordination of immune responses.

Biological Activity

Measured by its ability to chemoattract U87 cells. The ED50 for this effect is 21.43 ng/mL, corresponding to a specific activity is 4.67×104 U/mg.

  • Measured by its ability to chemoattract U87 cells. The ED50 for this effect is 21.43 ng/mL, corresponding to a specific activity is 4.67×104 U/mg.
Species

Rat

Source

HEK293

Tag

C-hFc

Accession

B4F7A5 (D25-G127)

Gene ID
Molecular Construction
N-term
CD99 (D25-G127)
Accession # B4F7A5
hFc
C-term
Protein Length

Partial

Synonyms
CD99 Antigen; 12E7; E2 Antigen; Protein MIC2; T-Cell Surface Glycoprotein E2; CD99; MIC2; MIC2X; MIC2Y
AA Sequence

DGDFRLDDALEDTDKKPTPKPPTPKKPSSGDFDLEEALTGGADEDPRRPGSRPKPDPKPPGPPRDSGGISDRDLEDVAGHGGRGGGAGDRGTDGAESEGQPQG

분자량

Approximately 48 kDa

Purity
  • ≥ 95%, as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

선적

Room temperature in continental US; may vary elsewhere.

각종 서류
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD99 Protein, Rat (HEK293, Fc)

 

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Inquiry Information

상품명:
CD99 Protein, Rat (HEK293, Fc)
Cat. No.:
HY-P75365
수량:
MCE Japan Authorized Agent: