CDCP1 Protein, Human (Biotinylated, HEK293, His-Avi)

CDCP1 protein may be involved in cell adhesion and extracellular matrix association. Phosphorylation of CDCP1 may regulate the balance between anchorage and migration, proliferation, and differentiation. CDCP1 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CDCP1 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

CDCP1 protein is potentially involved in cell adhesion and the association with the extracellular matrix. Its phosphorylation may play a role in regulating the balance between anchorage and migration, as well as proliferation and differentiation. Additionally, CDCP1 has shown promise as a novel marker for leukemia diagnosis and for identifying immature hematopoietic stem cell subsets. It belongs to the tetraspanin web, which is implicated in tumor progression and metastasis. CDCP1 interacts with several proteins including CDH2/N-cadherin, CDH3/P-cadherin, SDC1/syndecan-1, SDC4/syndecan-4, the serine protease ST14/MT-SP1, as well as SRC and PRKCG/protein kinase C gamma, suggesting its involvement in various cellular processes and signaling pathways.

1. Immobilized Biotinylated Human CDCP1 at 2 μg/mL (100 μL/Well) on the plate. Dose response curve for Anti-CDCP1 Antibody with the EC₅₀ < 10.1 ng/mL determined by ELISA.
2. Immobilized Anti-CDCP1 Antibody, hFc Tag at 1 μg/mL (100 μl/Well) on the plate. Dose response curve for Biotinylated Human CDCP1, His Tag with the EC₅₀ ≤ 80.3 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-8*His

Q9H5V8-1 (F30-L666)

64866  [NCBI]

Extracellular Domain

Biotin

The single lysine residue in Avitag is biotinylated through an enzymatic reaction.

FEIALPRESNITVLIKLGTPTLLAKPCYIVISKRHITMLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNRTFIWDVKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSRIKMQEGVKMALHLPWFHPRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPEGFPEDELMTWQFVVPAHLRASVSFLNFNLSNCERKEERVEYYIPGSTTNPEVFKLEDKQPGNMAGNFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNESNKIYVVDLSNERAMSLTIEPRPVKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCDDLTRLWMNVEKTISCTDHRYCQRKSYSLQVPSDILHLPVELHDFSWKLLVPKDRLSLVLVPAQKLQQHTHEKPCNTSFSYLVASAIPSQDLYFGSFCPGGSIKQIQVKQNISVTLRTFAPSFQQEASRQGLTVSFIPYFKEEGVFTVTPDTKSKVYLRTPNWDRGLPSLTSVSWNISVPRDQVACLTFFKERSGVVCQTGRAFMIIQEQRTRAEEIFSLDEDVLPKPSFHHHSFWVNISNCSPTSGKQLDLLFSVTLTPRTVDL

Approximately 85-100 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by Bis-Tris PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Qi X, et al. CDCP1: A promising diagnostic biomarker and therapeutic target for human cancer. Life Sci. 2022 Jul 15;301:120600.  [Content Brief]

  [2]. He Y, et al. CDCP1 enhances Wnt signaling in colorectal cancer promoting nuclear localization of β-catenin and E-cadherin. Oncogene. 2020 Jan;39(1):219-233.  [Content Brief]

  [3]. Kajiwara K, et al. CDCP1 promotes compensatory renal growth by integrating Src and Met signaling. Life Sci Alliance. 2021 Feb 11;4(4):e202000832.  [Content Brief]

  [4]. Conze T, et al. CDCP1 is a novel marker for hematopoietic stem cells. Ann N Y Acad Sci. 2003 May;996:222-6.  [Content Brief]

  [5]. Scherl-Mostageer M, et al. Identification of a novel gene, CDCP1, overexpressed in human colorectal cancer. Oncogene. 2001 Jul 19;20(32):4402-8.  [Content Brief]