CDK3-CCNE2 Protein, Human (sf9, GST, Flag, His)
CDK3 protein is a serine/threonine protein kinase that plays a key role in regulating the eukaryotic cell cycle, especially affecting the G0-G1 and G1-S transitions. It interacts with CCNC/cyclin-C during interphase and phosphorylates substrates such as histone H1, ATF1, RB1, and CABLES1. CDK3-CCNE2 Protein, Human (sf9, GST, Flag, His) is the recombinant human-derived CDK3-CCNE2, expressed by Sf9 insect cells, with N-His, N-GST, N-Flag labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
CDK3 protein is a serine/threonine protein kinase that plays a key role in regulating the eukaryotic cell cycle, especially affecting the G0-G1 and G1-S transitions. It interacts with CCNC/cyclin-C during interphase and phosphorylates substrates such as histone H1, ATF1, RB1, and CABLES1. CDK3-CCNE2 Protein, Human (sf9, GST, Flag, His) is the recombinant human-derived CDK3-CCNE2, expressed by Sf9 insect cells, with N-His, N-GST, N-Flag labeled tag.
Background
CDK3 Protein is a serine/threonine-protein kinase that assumes a pivotal role in the regulation of the eukaryotic cell cycle, specifically influencing G0-G1 and G1-S transitions. During interphase, it interacts with CCNC/cyclin-C. The kinase phosphorylates key substrates, including histone H1, ATF1, RB1, and CABLES1. ATF1 phosphorylation induced by CDK3-CCNE1 triggers ATF1 transactivation and transcriptional activities, consequently fostering cell proliferation and transformation. Moreover, the CDK3/cyclin-C-mediated phosphorylation of RB1 is essential for the G0-G1 transition. Notably, CDK3-CCNE1 contributes to the G1-S transition, potentially by participating in the activation of E2F1, E2F2, and E2F3 in a RB1-independent manner. This intricate interplay positions CDK3-CCNE1 as a crucial orchestrator of cell cycle progression.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His;N-GST;N-Flag
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Accession
Q00526 (M1-H305)&O96020-1 (S2-H404 )
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Gene ID1018&9134
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Synonyms
CDK3; Cyclin-Dependent Kinase 3; Cyclin Dependent Kinase 3; Cyclin-Dependent Kinase; Cell Division Protein Kinase 3; CDKN3; CCNE2; G1/S-Specific Cyclin-E2; Cyclin E2; Alternative Protein CCNE2; CYCE2; CCNE2 Protein
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Predicted Molecular Mass
61.6 kDa & 49.4 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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