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  4. Chymase/CMA1 Protein, Human (sf9, His)

Chymase/CMA1 Protein, Human (sf9, His)

Cat. No.: HY-P74252
COA Handling Instructions

Chymase/CMA1 Protein, a secreted protease from mast cells, plays a significant role in generating vasoactive peptides, degrading the extracellular matrix, and regulating gland secretion. Chymase/CMA1 Protein, Human (sf9, His) is the recombinant human-derived Chymase/CMA1 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Chymase/CMA1 Protein, Human (sf9, His) is 247 a.a., with molecular weight of ~33 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

Chymase/CMA1 Protein, a secreted protease from mast cells, plays a significant role in generating vasoactive peptides, degrading the extracellular matrix, and regulating gland secretion. Chymase/CMA1 Protein, Human (sf9, His) is the recombinant human-derived Chymase/CMA1 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Chymase/CMA1 Protein, Human (sf9, His) is 247 a.a., with molecular weight of ~33 kDa.

Background

Chymase/CMA1 protein is a significant secreted protease produced by mast cells, and it is believed to have various functions such as the generation of vasoactive peptides, degradation of the extracellular matrix, and regulation of gland secretion.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

Sf9 insect cells

Tag

C-His

Accession

P23946 (M1-N247)

Gene ID
Molecular Construction
N-term
CMA1 (M1-N247)
Accession # P23946
His
C-term
Synonyms
Alpha-chymase; Mast cell chymase 1; Mast cell protease 5; mMCP-5
Molecular Weight

Approximately 33 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% glycerol.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Chymase/CMA1 Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Chymase/CMA1 Protein, Human (sf9, His)
Cat. No.:
HY-P74252
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