Complement C5/C5a Protein, Mouse (Biotinylated)

Complement C5/C5a Protein activates late complement components to form the membrane attack complex, C5-C9. C5b binds C6, initiating lytic complex assembly. C5a, produced by C5 degradation, acts as an anaphylatoxin, inducing local inflammation. C5a stimulates intracellular calcium release, smooth muscle contraction, vascular permeability, histamine release, and acts as a chemokine, guiding leukocyte migration. Complement C5/C5a Protein, Mouse (Biotinylated) is the recombinant mouse-derived Complement C5/C5a protein, expressed by E. coli, with tag free. The total length of Complement C5/C5a Protein, Mouse (Biotinylated) is 77 a.a., with molecular weight of ~9 KDa.

Complement C5/C5a Protein, when activated by a C5 convertase, triggers the spontaneous assembly of the late complement components into the membrane attack complex, C5-C9. C5b forms a transient binding site for C6, forming the foundation for the assembly of the lytic complex. Meanwhile, C5a, produced through proteolytic degradation of complement C5, acts as an anaphylatoxin mediating local inflammatory processes. Its binding to the receptor C5AR1 induces various responses such as intracellular calcium release, smooth muscle contraction, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. Additionally, C5a acts as a potent chemokine, stimulating the movement of polymorphonuclear leukocytes and guiding their migration towards inflamed areas.

Mouse

E. coli

Tag Free

P06684 (N679-R755)

15139  [NCBI]

Approximately 9 kDa.

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.