CPA2 Protein, Mouse (HEK293, His)
CPA2 Protein is A member of the carboxypeptidase family that encodes zinc metalloproteinases and is expressed by pancreatic exocrine cells. CPA2 Protein acts on aromatic C-terminal residues and plays an important role in the process of degrading food to form amino acids. CPA2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CPA2 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Mouse
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
CPA2 Protein is A member of the carboxypeptidase family that encodes zinc metalloproteinases and is expressed by pancreatic exocrine cells. CPA2 Protein acts on aromatic C-terminal residues and plays an important role in the process of degrading food to form amino acids. CPA2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CPA2 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
Carboxypeptidase A2 (CPA2) is A member of the carboxypeptidase family that encodes zinc metalloproteinases. The encoded preproprotein is treated with proteolysis to remove the N-terminal activating peptide and produce a functional enzyme. At present, three different forms of human pancreatic procarboxypeptidase A have been isolated. A1 and A2 are monomer proteins with different biochemical properties. The A2 form of pancreatic procarboxypeptidase acts on aromatic C-terminal residues. Substrate Inhibition Inhibition of aromatic n-acyldipeptide substrates and their ester analogs Apparent CPA2 is expressed by pancreatic exocrine cells, which secrete this enzyme during digestion. This gene is located in the carboxypeptidase gene cluster on chromosome 6. CPA2 is important in the process of degrading food proteins leading to the formation of amino acids. CPA2 also plays a role in the renin-angiotensin system[1][2][3].
Technical Parameters
-
Species Mouse
-
Source HEK293
-
Tag C-6*His
-
Accession
Q504N0 (Q17-Y417)
-
Molecular Construction
-
N-term
-
CPA2 (Q17-Y417)
Accession # Q504N0 -
6*His
-
C-term
-
-
Protein Length
Full Length of Mature Protein
-
Synonyms
CPA2; Carboxypeptidase A2 Isoform 4; Carboxypeptidase A2; Carboxypeptidase A2 Isoform 1; Carboxypeptidase A2 (Pancreatic); Carboxypeptidase A2 Isoform 5
-
AA Sequence
QETFVGDQVLEVIPNDEEQIKTLLQLEAEEHLELDFWKSPSVPRQTVHVRVPFASIQDVKVFLESQGITYSIMIEDVQVLLDQEREEMLFNQQRERGTNFNFGAYHTLEEIYQEMDNLVAENPGLVSKVNIGSSFENRPMNVLKFSTGGDKPAIWLDAGIHAREWVTQATALWTANKIASDYGTDPAITSLLNTLDVFLLPVTNPDGYVFSQTSNRMWRKTRSKRSGSFCVGVDPNRNWDANFGGPGASSNPCSDSYHGPSPNSEVEVKSIVDFIKSHGKVKAFITLHSYSQLLMFPYGYKCAKPDDFNELDEVAQRAAQSLKRLHGTSYKVGPICSVIYQASGGSIDWAYDLGIKYSFAFELRDTGYYGFLLPAKQILPTAEETWLGLKTIMEHVRDHPY
-
Predicted Molecular Mass
46.2 kDa
-
Molecular Weight
Approximately 41-50 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
