CRELD1 Protein, Mouse (HEK293, His)

The CRELD1 protein is a member of the protein disulfide isomerase family and is actively involved in rearrangements of disulfide bonds that contribute to important cellular processes. Of particular note is the role of CRELD1 in promoting the localization of acetylcholine receptors (AChR) to the plasma membrane. CRELD1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CRELD1 protein, expressed by HEK293 , with C-His labeled tag. The total length of CRELD1 Protein, Mouse (HEK293, His) is 362 a.a., with molecular weight of ~40-50 KDa.

CRELD1 Protein, identified as a member of the protein disulfide isomerase family, plays a significant role in cellular processes. This protein, akin to other members of the family, is involved in disulfide bond rearrangement. Notably, CRELD1 has been found to facilitate the localization of acetylcholine receptors (AChRs) to the plasma membrane. This function underscores the importance of CRELD1 in the organization and positioning of key cellular components, particularly the acetylcholine receptors, highlighting its potential impact on cellular communication and signaling processes.

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.5602 μg/mL, corresponding to a specific activity is 1785.077 units/mg.

Mouse

HEK293

C-6*His

Q91XD7 (Q30-E362)

171508  [NCBI]

QPSPPPHPSPRAEPHPCHTCRALVDNFNKGLERTIRDNFGGGNTAWEEEKLSKYKDSETRLVEVLEGVCSRSDFECHRLLELSEELVENWWFHRQQEAPDLFQWLCSDSLKLCCPSGTFGPSCLPCPGGTERPCGGYGQCEGEGTRGGSGHCDCQAGYGGEACGQCGLGYFEAERNSSHLVCSACFGPCARCTGPEESHCLQCKKGWALHHLKCVDIDECGTEQATCGADQFCVNTEGSYECRDCAKACLGCMGAGPGRCKKCSRGYQQVGSKCLDVDECETVVCPGENEKCENTEGGYRCVCAEGYRQEDGICVKEQVPESAGFFAEMTEDE

Approximately 40-50 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.