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CRELD1 Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

The CRELD1 protein is a member of the protein disulfide isomerase family and is actively involved in rearrangements of disulfide bonds that contribute to important cellular processes. Of particular note is the role of CRELD1 in promoting the localization of acetylcholine receptors (AChR) to the plasma membrane. CRELD1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CRELD1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CRELD1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for CRELD1 Protein, Mouse (HEK293, His)
  • CRELD1 Protein, Mouse (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The CRELD1 protein is a member of the protein disulfide isomerase family and is actively involved in rearrangements of disulfide bonds that contribute to important cellular processes. Of particular note is the role of CRELD1 in promoting the localization of acetylcholine receptors (AChR) to the plasma membrane. CRELD1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CRELD1 protein, expressed by HEK293 , with C-His labeled tag.

Background

CRELD1 Protein, identified as a member of the protein disulfide isomerase family, plays a significant role in cellular processes. This protein, akin to other members of the family, is involved in disulfide bond rearrangement. Notably, CRELD1 has been found to facilitate the localization of acetylcholine receptors (AChRs) to the plasma membrane. This function underscores the importance of CRELD1 in the organization and positioning of key cellular components, particularly the acetylcholine receptors, highlighting its potential impact on cellular communication and signaling processes.

Verified Bioactivity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.5602 μg/mL, corresponding to a specific activity is 1785.077 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.5602 μg/mL, corresponding to a specific activity is 1785.077 units/mg.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q91XD7 (Q30-E362)

  • Gene ID
    171508  [NCBI]
  • Molecular Construction
    • N-term
    • CRELD1 (Q30-E362)
      Accession # Q91XD7
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CRELD1; Cysteine-Rich With EGF-Like Domain Protein 1; Prev. AVSD2; Atrioventricular Septal Defect 2; Cysteine Rich With EGF Like Domains 1; Protein Disulfide-Isomerase; CIRRIN; JELANS; CRELD Disulfide Isomerase 1; Protein Disulfide Isomerase CRELD1

  • AA Sequence

    QPSPPPHPSPRAEPHPCHTCRALVDNFNKGLERTIRDNFGGGNTAWEEEKLSKYKDSETRLVEVLEGVCSRSDFECHRLLELSEELVENWWFHRQQEAPDLFQWLCSDSLKLCCPSGTFGPSCLPCPGGTERPCGGYGQCEGEGTRGGSGHCDCQAGYGGEACGQCGLGYFEAERNSSHLVCSACFGPCARCTGPEESHCLQCKKGWALHHLKCVDIDECGTEQATCGADQFCVNTEGSYECRDCAKACLGCMGAGPGRCKKCSRGYQQVGSKCLDVDECETVVCPGENEKCENTEGGYRCVCAEGYRQEDGICVKEQVPESAGFFAEMTEDE

  • Molecular Weight

    Approximately 48 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.5602 μg/mL, corresponding to a specific activity is 1785.077 units/mg.

  • CRELD1 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CRELD1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for CRELD1 Protein, Mouse (HEK293, His)
  • CRELD1 Protein, Mouse (HEK293, His) Product Image