CSK Protein, Human (Active, sf9, GST)
Based on 1 Customer Validation
CSK is a non-receptor tyrosine protein kinase that critically regulates cell growth, differentiation, migration and immune responses by phosphorylating tyrosine residues in the C-terminal tail of Src family kinases (SFK). This induces an inactive conformation through intramolecular interactions with the SH2 domain of SFK. CSK Protein, Human (Active, sf9, GST) is the recombinant human-derived CSK protein, expressed by Sf9 insect cells , with N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
CSK is a non-receptor tyrosine protein kinase that critically regulates cell growth, differentiation, migration and immune responses by phosphorylating tyrosine residues in the C-terminal tail of Src family kinases (SFK). This induces an inactive conformation through intramolecular interactions with the SH2 domain of SFK. CSK Protein, Human (Active, sf9, GST) is the recombinant human-derived CSK protein, expressed by Sf9 insect cells , with N-GST labeled tag.
Background
CSK, a non-receptor tyrosine-protein kinase, plays a crucial role in regulating cell growth, differentiation, migration, and immune response. It phosphorylates tyrosine residues within the C-terminal tails of Src-family kinases (SFKs), including LCK, SRC, HCK, FYN, LYN, CSK, or YES1. This phosphorylation triggers intramolecular interactions between the phosphotyrosine tail and the SH2 domain of SFKs, inducing an inactive conformation. To exert its inhibitory function on SFKs, CSK is recruited to the plasma membrane through interactions with transmembrane or adapter proteins in close proximity to the membrane. CSK effectively suppresses signaling from various surface receptors, such as the T-cell receptor (TCR) and B-cell receptor (BCR), by phosphorylating and maintaining several positive effectors, including FYN or LCK, in an inactive state.
Verified Bioactivity
The specific activity was determined to be >50 nmol/min/mg using Poly(Glu,Tyr) 4:1 as substrate.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-GST
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Accession
P41240 (M1-L450)
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Molecular Construction
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N-term
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GST
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CSK (M1-L450)
Accession # P41240 -
C-term
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Protein Length
Full Length
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Synonyms
CSK; Protein-Tyrosine Kinase CYL; C-Terminal Src Kinase; C-Src Tyrosine Kinase; CSK, Non-Receptor Tyrosine Kinase; C-Src Kinase; Tyrosine-Protein Kinase CSK; Tyrosine-Protein Kinase
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AA Sequence
MSAIQAAWPSGTECIAKYNFHGTAEQDLPFCKGDVLTIVAVTKDPNWYKAKNKVGREGIIPANYVQKREGVKAGTKLSLMPWFHGKITREQAERLLYPPETGLFLVRESTNYPGDYTLCVSCDGKVEHYRIMYHASKLSIDEEVYFENLMQLVEHYTSDADGLCTRLIKPKVMEGTVAAQDEFYRSGWALNMKELKLLQTIGKGEFGDVMLGDYRGNKVAVKCIKNDATAQAFLAEASVMTQLRHSNLVQLLGVIVEEKGGLYIVTEYMAKGSLVDYLRSRGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSEDNVAKVSDFGLTKEASSTQDTGKLPVKWTAPEALREKKFSTKSDVWSFGILLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYEVMKNCWHLDAAMRPSFLQLREQLEHIKTHELHL
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Predicted Molecular Mass
77 kDa
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Molecular Weight
Approximately 66 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.5, 25% glycerol, 0.5 mM TCEP, 0.25 mM GSH.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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