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CTLA-4 Protein, Guinea pig (P.pastoris, His)

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Based on 1 Customer Validation

CTLA-4 protein, a potent inhibitory receptor, plays a crucial role in suppressing T-cell responses by binding strongly to its ligands CD80 and CD86, surpassing the affinity of CD28. This heightened affinity allows CTLA-4 to act as a major negative regulator, maintaining immune homeostasis and preventing excessive T-cell activation. The balance between CTLA-4 and its ligands is pivotal for immune regulation. CTLA-4 Protein, Guinea pig (P.pastoris, His) is the recombinant CTLA-4 protein, expressed by P. pastoris , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Others
  • Source: P. pastoris
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for CTLA-4 Protein, Guinea pig (P.pastoris, His)
  • CTLA-4 Protein, Guinea pig (P.pastoris, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

CTLA-4 protein, a potent inhibitory receptor, plays a crucial role in suppressing T-cell responses by binding strongly to its ligands CD80 and CD86, surpassing the affinity of CD28. This heightened affinity allows CTLA-4 to act as a major negative regulator, maintaining immune homeostasis and preventing excessive T-cell activation. The balance between CTLA-4 and its ligands is pivotal for immune regulation. CTLA-4 Protein, Guinea pig (P.pastoris, His) is the recombinant CTLA-4 protein, expressed by P. pastoris , with C-6*His labeled tag.

Background

CTLA-4 protein functions as a prominent inhibitory receptor, playing a crucial role in dampening T-cell responses. Its inhibitory action stems from the remarkably strong affinity CTLA-4 exhibits for its natural B7 family ligands, CD80 and CD86, surpassing the affinity of their counterpart stimulatory coreceptor, CD28. By virtue of this heightened affinity, CTLA-4 serves as a major negative regulator in the intricate orchestration of T-cell activation and immune responses. The nuanced balance between stimulatory and inhibitory signals mediated by CTLA-4 and its ligands is pivotal for maintaining immune homeostasis and preventing excessive or aberrant T-cell activation.

Verified Bioactivity

The ED50 as determined by its ability to bind Mouse B7-1 in functional ELISA is less than 0.4 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Others
  • Source P. pastoris
  • Tag C-6*His
  • Accession

    H0VUB1 (A37-D161)

  • Gene ID
    100725760  [NCBI]
  • Molecular Construction
    • N-term
    • CTLA-4 (A37-D161)
      Accession # H0VUB1
    • 6*His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    CTLA4; Celiac Disease 3; Prev. CELIAC3; Cytotoxic T Lymphocyte Associated Antigen 4 Short Spliced Form; Prev. IDDM12; Cytotoxic T-Lymphocyte-Associated Serine Esterase-4; CTLA-4; Cytotoxic T-Lymphocyte-Associated Protein 4; CD152; Cytotoxic T-Lymphocyte A

  • AA Sequence

    AMHVAQPAVVLASSRGVASFECEYASSHNANEVRVTVLQQVASRTTEICAATYTVERELAFPEDSACAGTSSGTRVNLTIQGLRAADTGLYICKVELMYPPPYFVGTGNGTQIYVIDPEPCPDSD

  • Predicted Molecular Mass

    16-35 kDa

  • Molecular Weight

    Approximately 16-35 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • CTLA-4 Protein, Guinea pig (P.pastoris, His) Product Image
    1/16
  • Experimental Validation Results for CTLA-4 Protein, Guinea pig (P.pastoris, His)
  • CTLA-4 Protein, Guinea pig (P.pastoris, His) Product Image