CUL1-RBX1 Protein, Human (Active, sf9, His, Strep)
CUL1 (the gene encoding cullin 1) and RBX1 are overexpressed in various cancers and are associated with poorer overall survival. CUL1-RBX1 Protein, Human (Active, sf9, His, Strep) is a recombinant human CUL1-RBX1 protein expressed by sf9 and tagged with His and Strep.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
CUL1 (the gene encoding cullin 1) and RBX1 are overexpressed in various cancers and are associated with poorer overall survival. CUL1-RBX1 Protein, Human (Active, sf9, His, Strep) is a recombinant human CUL1-RBX1 protein expressed by sf9 and tagged with His and Strep.
Background
The SCF complexes are RING-type E3s that consist of Cul1 (776 residues), Rbx1 (108 residues), Skp1 (163 residues) and a member of the F-box protein family (∼430 to >1,000 residues). Rbx1, which contains the RING domain, and Cul1 form a catalytic core complex that recruits a cognate E2, the variable F-box protein subunit binds the substrate, and Skp1 serves as an adapter that links the F-box protein to Cul1. Homology to the Cul1 and Rbx1 SCF subunits has also been found in the 11-subunit Anaphase-Promoting Complex (APC) E3 that promotes the ubiquitination of several mitotic regulatory proteins.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag Strep;His
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Accession
Q13616 (S2-A776)&P62877 (M1-H108)
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Gene ID8454
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Synonyms
CUL1; Cullin-1; Cullin 1; CUL-1; RBX1; E3 Ubiquitin-Protein Ligase RBX1; Ring-Box 1; RING Finger Protein 75; RNF75; RING-Box Protein 1; ROC1; Ring-Box 1, E3 Ubiquitin Protein Ligase; Regulator Of Cullins 1; ZYP Protein; BA554C12.1; Protein ZYP; E3 Ubiquit
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Predicted Molecular Mass
92.7 kDa & 12.2 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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