DHCR7 Protein, Bovine (Cell-Free, His)
DHCR7 Protein, alias 7-dehydrocholesterol reductase, crucially participates in the cholesterol biosynthetic pathway by reducing the C7-C8 double bond in intermediates—cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol/7-DHC) and cholesta-5,7,24-trien-3beta-ol. DHCR7 Protein, Bovine (Cell-Free, His) is the recombinant bovine-derived DHCR7 protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
- Species: Bovine
- Source: E. coli Cell-free
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
DHCR7 Protein, alias 7-dehydrocholesterol reductase, crucially participates in the cholesterol biosynthetic pathway by reducing the C7-C8 double bond in intermediates—cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol/7-DHC) and cholesta-5,7,24-trien-3beta-ol. DHCR7 Protein, Bovine (Cell-Free, His) is the recombinant bovine-derived DHCR7 protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
Background
The DHCR7 Protein, also known as 7-dehydrocholesterol reductase, is an enzyme involved in the cholesterol biosynthetic pathway. It plays a crucial role in reducing the C7-C8 double bond of two intermediates in the pathway, namely cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol/7-DHC) and cholesta-5,7,24-trien-3beta-ol.
Technical Parameters
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Species Bovine
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Source E. coli Cell-free
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Tag N-10*His
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Accession
Q5E9J5 (M1-F475)
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Gene ID514745
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Molecular Construction
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N-term
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10*His
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DHCR7 (M1-F475)
Accession # Q5E9J5 -
C-term
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Protein Length
Full Length
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Synonyms
DHCR7; Delta-7-Dehydrocholesterol Reductase; Prev. SLOS; Delta(14)-Sterol Reductase TM7SF2; Cholesterol-5,6-Epoxide Hydrolase Subunit DHCR7; Putative Sterol Reductase SR-2; Sterol Delta(7)-Reductase; Smith-Lemli-Opitz Syndrome; Delta7-Sterol Reductase; St
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AA Sequence
MAAKSQPSAPKTKSTSGLTNGNAAAQGQWGRAWEVDWFSLASVIFLLLFAPFIVYYFIMACDQYGCSLTVPVADLATGRARLADIWARTPPVTAKAAQIYTAWVTLQVLLYMLLPDFCHKFLPGYVGGVQEGAVTPAGAVNKYEINGLQAWLLTHLLWFANAHLLGWFSPTIIFDNWIPLLWCANILGYTVSTFAMVKGYLFPTDARECKFTGNFFYNYMMGVEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQQELYGHVTNSMVLVNILQAIYVLDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLPTYYALGVLLLGLLGYYIFRMTNHQKDLFRRTDGRCLIWGRKPKAIECSYTSADGQRHHSKLLVSGFWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIFMAILLTHRCLRDEHRCANKYGRDWEHYTAAVPYRLLPGIF
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Predicted Molecular Mass
55.8 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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