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  5. DHCR7 Protein, Bovine (Cell-Free, His)

DHCR7 Protein, Bovine (Cell-Free, His)

Cat. No.: HY-P702263
Handling Instructions

DHCR7 Protein, alias 7-dehydrocholesterol reductase, crucially participates in the cholesterol biosynthetic pathway by reducing the C7-C8 double bond in intermediates—cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol/7-DHC) and cholesta-5,7,24-trien-3beta-ol. DHCR7 Protein, Bovine (Cell-Free, His) is the recombinant bovine-derived DHCR7 protein, expressed by E. coli Cell-free, with N-10*His labeled tag. The total length of DHCR7 Protein, Bovine (Cell-Free, His) is 475 a.a., with molecular weight of 55.8 kDa.

For research use only. We do not sell to patients.

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Description

DHCR7 Protein, alias 7-dehydrocholesterol reductase, crucially participates in the cholesterol biosynthetic pathway by reducing the C7-C8 double bond in intermediates—cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol/7-DHC) and cholesta-5,7,24-trien-3beta-ol. DHCR7 Protein, Bovine (Cell-Free, His) is the recombinant bovine-derived DHCR7 protein, expressed by E. coli Cell-free, with N-10*His labeled tag. The total length of DHCR7 Protein, Bovine (Cell-Free, His) is 475 a.a., with molecular weight of 55.8 kDa.
Background

The DHCR7 Protein, also known as 7-dehydrocholesterol reductase, is an enzyme involved in the cholesterol biosynthetic pathway. It plays a crucial role in reducing the C7-C8 double bond of two intermediates in the pathway, namely cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol/7-DHC) and cholesta-5,7,24-trien-3beta-ol.
Species

Bovine
Source

E. coli Cell-free
Tag

N-10*His
Accession

Q5E9J5 (M1-F475)
Gene ID

514745
Molecular Construction
N-term
10*His
DHCR7 (M1-F475)
Accession # Q5E9J5
C-term
Synonyms
7-dehydrocholesterol reductase; Sterol Delta(7)-reductase
AA Sequence

MAAKSQPSAPKTKSTSGLTNGNAAAQGQWGRAWEVDWFSLASVIFLLLFAPFIVYYFIMACDQYGCSLTVPVADLATGRARLADIWARTPPVTAKAAQIYTAWVTLQVLLYMLLPDFCHKFLPGYVGGVQEGAVTPAGAVNKYEINGLQAWLLTHLLWFANAHLLGWFSPTIIFDNWIPLLWCANILGYTVSTFAMVKGYLFPTDARECKFTGNFFYNYMMGVEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQQELYGHVTNSMVLVNILQAIYVLDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLPTYYALGVLLLGLLGYYIFRMTNHQKDLFRRTDGRCLIWGRKPKAIECSYTSADGQRHHSKLLVSGFWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIFMAILLTHRCLRDEHRCANKYGRDWEHYTAAVPYRLLPGIF

Molecular Weight

55.8 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

DHCR7 Protein, Bovine (Cell-Free, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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