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Dipeptidyl peptidase 3/DPP3 Protein, Human (His)

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Based on 1 Customer Validation

According to research reports, dipeptidyl peptidase 3 (DPP3) protein, as an enzymatic entity, is able to cleave and degrade various bioactive peptides, including substrates such as angiotensin, leucine-enkephalin, and methionine-enkephalin. According to the results of multiple studies, in addition to its role in processing these bioactive peptides, DPP3 also has the ability to cleave Arg-Arg-β-naphthylamide in vitro. Dipeptidyl peptidase 3/DPP3 Protein, Human (His) is the recombinant human-derived Dipeptidyl peptidase 3/DPP3 protein, expressed by E. coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Dipeptidyl peptidase 3/DPP3 Protein, Human (His)
  • Experimental Validation Results for Dipeptidyl peptidase 3/DPP3 Protein, Human (His)
  • Dipeptidyl peptidase 3/DPP3 Protein, Human (His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

According to research reports, dipeptidyl peptidase 3 (DPP3) protein, as an enzymatic entity, is able to cleave and degrade various bioactive peptides, including substrates such as angiotensin, leucine-enkephalin, and methionine-enkephalin. According to the results of multiple studies, in addition to its role in processing these bioactive peptides, DPP3 also has the ability to cleave Arg-Arg-β-naphthylamide in vitro. Dipeptidyl peptidase 3/DPP3 Protein, Human (His) is the recombinant human-derived Dipeptidyl peptidase 3/DPP3 protein, expressed by E. coli , with N-6*His labeled tag.

Background

Dipeptidyl peptidase 3 (DPP3) is a protein with significant roles in peptide metabolism, cleaving and degrading various bioactive peptides, including angiotensin, Leu-enkephalin, and Met-enkephalin. This enzymatic activity suggests its involvement in the regulation of key physiological processes, particularly those related to blood pressure and pain modulation. Additionally, DPP3 has been shown to cleave Arg-Arg-beta-naphthylamide in vitro, further expanding its substrate specificity. The diverse range of peptides targeted by DPP3 underscores its importance in modulating the levels of signaling molecules and highlights its potential implications in various physiological and pathological contexts (

Verified Bioactivity

1.Measured by its ability to cleave the fluorogenic peptide substrate, Arg-Arg-7-amido-4-methylcoumarin (RR-AMC, HY-P4340) that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 2417.68 pmol/min/μg.
2.Loaded Procizumab (HY-P991050) on ProA biosensor, can bind Dipeptidyl peptidase 3/DPP3 Protein, Human (His) with an affinity constant of 7.400E-09 M as determined in BLI assay.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - BLI

    Bioactivity - BLI

    Loaded Procizumab (HY-P991050) on ProA biosensor, can bind Dipeptidyl peptidase 3/DPP3 Protein, Human (His) with an affinity constant of 7.400E-09 M as determined in BLI assay.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His
  • Accession

    Q9NY33 (M1-A737)

  • Gene ID
    10072  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • DPP3 (M1-A737)
      Accession # Q9NY33
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    DPP3; DPP III; Dipeptidyl Peptidase 3; Dipeptidylpeptidase III; Dipeptidyl Aminopeptidase III; Dipeptidyl-Peptidase 3; Dipeptidyl Peptidase III; Dipeptidylpeptidase 3; Enkephalinase B; DPPIII; Dipeptidyl Arylamidase III

  • AA Sequence

    MADTQYILPNDIGVSSLDCREAFRLLSPTERLYAYHLSRAAWYGGLAVLLQTSPEAPYIYALLSRLFRAQDPDQLRQHALAEGLTEEEYQAFLVYAAGVYSNMGNYKSFGDTKFVPNLPKEKLERVILGSEAAQQHPEEVRGLWQTCGELMFSLEPRLRHLGLGKEGITTYFSGNCTMEDAKLAQDFLDSQNLSAYNTRLFKEVDGEGKPYYEVRLASVLGSEPSLDSEVTSKLKSYEFRGSPFQVTRGDYAPILQKVVEQLEKAKAYAANSHQGQMLAQYIESFTQGSIEAHKRGSRFWIQDKGPIVESYIGFIESYRDPFGSRGEFEGFVAVVNKAMSAKFERLVASAEQLLKELPWPPTFEKDKFLTPDFTSLDVLTFAGSGIPAGINIPNYDDLRQTEGFKNVSLGNVLAVAYATQREKLTFLEEDDKDLYILWKGPSFDVQVGLHELLGHGSGKLFVQDEKGAFNFDQETVINPETGEQIQSWYRSGETWDSKFSTIASSYEECRAESVGLYLCLHPQVLEIFGFEGADAEDVIYVNWLNMVRAGLLALEFYTPEAFNWRQAHMQARFVILRVLLEAGEGLVTITPTTGSDGRPDARVRLDRSKIRSVGKPALERFLRRLQVLKSTGDVAGGRALYEGYATVTDAPPECFLTLRDTVLLRKESRKLIVQPNTRLEGSDVQLLEYEASAAGLIRSFSERFPEDGPELEEILTQLATADARFWKGPSEAPSGQA

  • Predicted Molecular Mass

    84.8 kDa

  • Molecular Weight

    Approximately 70-90 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 25 mM Tris-HCl, 10 mM NaCl, 2 mM DTT, 0.05% Tween 80, 20% Glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Loaded Procizumab (HY-P991050) on ProA biosensor, can bind Dipeptidyl peptidase 3/DPP3 Protein, Human (His) with an affinity constant of 7.400E-09 M as determined in BLI assay.

  • Dipeptidyl peptidase 3/DPP3 Protein, Human (His) Product Image
    1/16
  • Experimental Validation Results for Dipeptidyl peptidase 3/DPP3 Protein, Human (His)
  • Experimental Validation Results for Dipeptidyl peptidase 3/DPP3 Protein, Human (His)
  • Dipeptidyl peptidase 3/DPP3 Protein, Human (His) Product Image