E-Selectin/CD62E Protein, Mouse (HEK293, His)

E-Selectin/CD62E protein, a cell-surface glycoprotein, mediates the adhesion of blood neutrophils to cytokine-activated endothelium through interaction with SELPLG/PSGL1. It also potentially contributes to capillary morphogenesis and interacts with SELPLG/PSGL1 and PODXL2. These multifaceted interactions highlight E-Selectin's role in cell adhesion and its potential influence on vascular development. E-Selectin/CD62E Protein, Mouse (HEK293, His) is the recombinant mouse-derived E-Selectin/CD62E protein, expressed by HEK293 , with C-His, C-10*His labeled tag. The total length of E-Selectin/CD62E Protein, Mouse (HEK293, His) is 536 a.a., with molecular weight of 70-110 kDa.

E-Selectin/CD62E protein, a cell-surface glycoprotein, plays a crucial role in immunoadhesion by mediating the adhesion of blood neutrophils to cytokine-activated endothelium through interaction with SELPLG/PSGL1. Beyond its involvement in immune responses, E-Selectin may also contribute to capillary morphogenesis. The protein interacts with SELPLG/PSGL1 and PODXL2 through the sialyl Lewis X epitope, with SELPLG sulfation seemingly not required for this interaction. These interactions highlight the multifaceted roles of E-Selectin in mediating cell adhesion events and potentially influencing processes related to vascular development.

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10⁴ cells/well are added to mouse E-Selectin/Fc Chimera coated plates (2 µg/mL with 100 µL/well), approximately 99.43% will adhere for 1 hour incubation at room temperature.

Mouse

HEK293

C-10*His

Q00690/NP_035475.1 (W29-P564)

20339  [NCBI]

WYYNASSELMTYDEASAYCQRDYTHLVAIQNKEEINYLNSNLKHSPSYYWIGIRKVNNVWIWVGTGKPLTEEAQNWAPGEPNNKQRNEDCVEIYIQRTKDSGMWNDERCNKKKLALCYTASCTNASCSGHGECIETINSYTCKCHPGFLGPNCEQAVTCKPQEHPDYGSLNCSHPFGPFSYNSSCSFGCKRGYLPSSMETTVRCTSSGEWSAPAPACHVVECEALTHPAHGIRKCSSNPGSYPWNTTCTFDCVEGYRRVGAQNLQCTSSGIWDNETPSCKAVTCDAIPQPQNGFVSCSHSTAGELAFKSSCNFTCEQSFTLQGPAQVECSAQGQWTPQIPVCKAVQCEALSAPQQGNMKCLPSASGPFQNGSSCEFSCEEGFELKGSRRLQCGPRGEWDSKKPTCSAVKCDDVPRPQNGVMECAHATTGEFTYKSSCAFQCNEGFSLHGSAQLECTSQGKWTQEVPSCQVVQCPSLDVPGKMNMSCSGTAVFGTVCEFTCPDDWTLNGSAVLTCGATGRWSGMPPTCEAPVSPTRP

70-110 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.