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E-Selectin/CD62E Protein, Mouse (HEK293, His)

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Based on 1 publication(s) in Google Scholar

E-Selectin/CD62E protein, a cell-surface glycoprotein, mediates the adhesion of blood neutrophils to cytokine-activated endothelium through interaction with SELPLG/PSGL1. It also potentially contributes to capillary morphogenesis and interacts with SELPLG/PSGL1 and PODXL2. These multifaceted interactions highlight E-Selectin's role in cell adhesion and its potential influence on vascular development. E-Selectin/CD62E Protein, Mouse (HEK293, His) is the recombinant mouse-derived E-Selectin/CD62E protein, expressed by HEK293 , with C-His, C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • E-Selectin/CD62E Protein, Mouse (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

E-Selectin/CD62E protein, a cell-surface glycoprotein, mediates the adhesion of blood neutrophils to cytokine-activated endothelium through interaction with SELPLG/PSGL1. It also potentially contributes to capillary morphogenesis and interacts with SELPLG/PSGL1 and PODXL2. These multifaceted interactions highlight E-Selectin's role in cell adhesion and its potential influence on vascular development. E-Selectin/CD62E Protein, Mouse (HEK293, His) is the recombinant mouse-derived E-Selectin/CD62E protein, expressed by HEK293 , with C-His, C-10*His labeled tag.

Background

E-Selectin/CD62E protein, a cell-surface glycoprotein, plays a crucial role in immunoadhesion by mediating the adhesion of blood neutrophils to cytokine-activated endothelium through interaction with SELPLG/PSGL1. Beyond its involvement in immune responses, E-Selectin may also contribute to capillary morphogenesis. The protein interacts with SELPLG/PSGL1 and PODXL2 through the sialyl Lewis X epitope, with SELPLG sulfation seemingly not required for this interaction. These interactions highlight the multifaceted roles of E-Selectin in mediating cell adhesion events and potentially influencing processes related to vascular development.

Verified Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to mouse E-Selectin/Fc Chimera coated plates (2 µg/mL with 100 µL/well), approximately 99.43% will adhere for 1 hour incubation at room temperature.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-10*His
  • Accession

    NP_035475.1 (W29-P564)

  • Gene ID
    20339  [NCBI]
  • Molecular Construction
    • N-term
    • E-Selectin/CD62E (W29-P564)
      Accession # NP_035475.1
    • 10*His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    SELE; ESEL; Prev. ELAM1; Endothelial Adhesion Molecule 1; Prev. ELAM; ELAM-1; Endothelial Leukocyte Adhesion Molecule 1; LECAM2; CD62 Antigen-Like Family Member E; Leukocyte Endothelial Cell Adhesion Molecule 2; E-Selectin; CD62E Antigen; CD62E; Selectin-

  • AA Sequence

    WYYNASSELMTYDEASAYCQRDYTHLVAIQNKEEINYLNSNLKHSPSYYWIGIRKVNNVWIWVGTGKPLTEEAQNWAPGEPNNKQRNEDCVEIYIQRTKDSGMWNDERCNKKKLALCYTASCTNASCSGHGECIETINSYTCKCHPGFLGPNCEQAVTCKPQEHPDYGSLNCSHPFGPFSYNSSCSFGCKRGYLPSSMETTVRCTSSGEWSAPAPACHVVECEALTHPAHGIRKCSSNPGSYPWNTTCTFDCVEGYRRVGAQNLQCTSSGIWDNETPSCKAVTCDAIPQPQNGFVSCSHSTAGELAFKSSCNFTCEQSFTLQGPAQVECSAQGQWTPQIPVCKAVQCEALSAPQQGNMKCLPSASGPFQNGSSCEFSCEEGFELKGSRRLQCGPRGEWDSKKPTCSAVKCDDVPRPQNGVMECAHATTGEFTYKSSCAFQCNEGFSLHGSAQLECTSQGKWTQEVPSCQVVQCPSLDVPGKMNMSCSGTAVFGTVCEFTCPDDWTLNGSAVLTCGATGRWSGMPPTCEAPVSPTRP

  • Molecular Weight

    Approximately 70-110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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