EGFR Protein, Mouse (HEK293, His)

The EGFR protein is a receptor tyrosine kinase that binds EGF family ligands and activates multiple signaling cascades. Ligand binding induces receptor homo- or heterodimerization, leading to autophosphorylation and recruitment of adapter proteins (such as GRB2). EGFR Protein, Mouse (HEK293, His) is the recombinant mouse-derived EGFR protein, expressed by HEK293 , with C-His labeled tag. The total length of EGFR Protein, Mouse (HEK293, His) is 647 a.a., with molecular weight of ~100 kDa.

EGFR Protein, a receptor tyrosine kinase, binds ligands of the EGF family, such as EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG, and HBEGF/heparin-binding EGF, activating multiple signaling cascades to transduce extracellular cues into cellular responses. Ligand binding induces receptor homo- and/or heterodimerization, leading to autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2, initiating downstream signaling cascades, including RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC, and STATs modules. EGFR may also activate the NF-kappa-B signaling cascade and directly phosphorylate proteins like RGS16, modulating G protein-coupled receptor signaling. Additionally, EGFR phosphorylates MUC1, enhancing its interaction with SRC and CTNNB1/beta-catenin. Notably, it positively regulates cell migration through interaction with CCDC88A/GIV, promoting EGFR signaling and triggering cell migration. EGFR also plays a role in enhancing learning and memory performance and contributes to mammalian pain signaling, leading to long-lasting hypersensitivity.

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2. Measured by its binding ability in a functional ELISA.
2.1. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind human EGF-Fc, The EC₅₀ of human EGF-Fc is 60-90 ng/mL.
2.2. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind mouse EGF-Fc, The EC₅₀ of mouse EGF-Fc is 70-100 ng/mL.

Mouse

HEK293

C-His

Q01279 (M1-S647)

13649  [NCBI]

MRPSGTARTTLLVLLTALCAAGGALEEKKVCQGTSNRLTQLGTFEDHFLSLQRMYNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNALYENTYALAILSNYGTNRTGLRELPMRNLQEILIGAVRFSNNPILCNMDTIQWRDIVQNVFMSNMSMDLQSHPSSCPKCDPSCPNGSCWGGGEENCQKLTKIICAQQCSHRCRGRSPSDCCHNQCAAGCTGPRESDCLVCQKFQDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGPDYYEVEEDGIRKCKKCDGPCRKVCNGIGIGEFKDTLSINATNIKHFKYCTAISGDLHILPVAFKGDSFTRTPPLDPRELEILKTVKEITGFLLIQAWPDNWTDLHAFENLEIIRGRTKQHGQFSLAVVGLNITSLGLRSLKEISDGDVIISGNRNLCYANTINWKKLFGTPNQKTKIMNNRAEKDCKAVNHVCNPLCSSEGCWGPEPRDCVSCQNVSRGRECVEKCNILEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGIMGENNTLVWKYADANNVCHLCHANCTYGCAGPGLQGCEVWPSGPKIPS

Approximately 100 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.