ELK1 Protein, Human (sf9, His-GST)

The ELK1 protein is a transcription factor that binds to purine-rich DNA and forms a ternary complex with SRF on the serum response element. It induces transcription, especially upon stimulation of the JNK signaling pathway. ELK1 Protein, Human (sf9, His-GST) is the recombinant human-derived ELK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of ELK1 Protein, Human (sf9, His-GST) is 428 a.a., with molecular weight of ~73 kDa.

The ELK1 protein serves as a transcription factor with an affinity for binding purine-rich DNA sequences. It forms a ternary complex with SRF and the ETS and SRF motifs present in the serum response element (SRE) on the promoter region of immediate early genes, including FOS and IER2. ELK1 induces the transcription of target genes, particularly in response to stimulation of the JNK-signaling pathway. In its sumoylated form, ELK1 interacts with PIAS2/PIASX, thereby enhancing its transcriptional activator activity. Additionally, ELK1 engages in a direct interaction with MAD2L2, promoting phosphorylation by the kinases MAPK8 and/or MAPK9. Furthermore, ELK1 interacts with POU1F1, highlighting its diverse associations in cellular processes and regulatory networks.

Human

Sf9 insect cells

N-His;N-GST

P19419 (M1-P428)

2002  [NCBI]

Approximately 73 kDa

Greater than 85% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.