ENPP-3 Protein, Rhesus Macaque (sf9, His)
ENPP-3 is a hydrolase that metabolizes extracellular nucleotides (ATP, GTP, UTP, CTP), which is critical for immune regulation of inflammation and chronic allergic reactions. By eliminating extracellular ATP, ENPP-3 inhibits basophil and mast cell activation and suppresses the release of inflammatory cytokines. ENPP-3 Protein, Rhesus Macaque (sf9, His) is the recombinant Rhesus Macaque-derived ENPP-3 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Species: Rhesus Macaque
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
ENPP-3 is a hydrolase that metabolizes extracellular nucleotides (ATP, GTP, UTP, CTP), which is critical for immune regulation of inflammation and chronic allergic reactions. By eliminating extracellular ATP, ENPP-3 inhibits basophil and mast cell activation and suppresses the release of inflammatory cytokines. ENPP-3 Protein, Rhesus Macaque (sf9, His) is the recombinant Rhesus Macaque-derived ENPP-3 protein, expressed by Sf9 insect cells , with N-His labeled tag.
Background
ENPP-3, a hydrolase, exhibits the ability to metabolize various extracellular nucleotides, including ATP, GTP, UTP, and CTP. This enzymatic activity plays a crucial role in modulating immune responses, particularly during inflammation and the chronic phases of allergic reactions. ENPP-3 acts by eliminating extracellular ATP, a signaling molecule that activates basophils and mast cells, leading to the release of inflammatory cytokines. Moreover, in the lumen of the small intestine, ENPP-3 metabolizes extracellular ATP, thereby preventing ATP-induced apoptosis of intestinal plasmacytoid dendritic cells. Besides its involvement in nucleotide metabolism, ENPP-3 also exhibits alkaline phosphodiesterase activity, adding to its multifaceted role in cellular processes.
Technical Parameters
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Species Rhesus Macaque
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Source Sf9 insect cells
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Tag N-His
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Accession
XP_001103528 (R46-I874)
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Molecular Construction
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N-term
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His
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ENPP-3 (R46-I874)
Accession # XP_001103528 -
C-term
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Protein Length
Partial
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Synonyms
ENPP3; B10; Prev. PDNP3; NPPase; Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 3; NPP3; Phosphodiesterase I/Nucleotide Pyrophosphatase 3; DJ334I18.1 (Ectonucleotide Pyrophosphatase/Phosphodiesterase 3); Alkaline Phosphodiesterase I; DJ100
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AA Sequence
RKLEKQGSCRKKCFDASFRGLENCRCDVACKDRGDCCWDFEDTCVASTRIWTCNKFRCGETRLEASLCSCSDDCLQRKDCCADYKSVCQGETSWLEENCDTAQQSQCPEGFDLPPVILFSMDGFRAEYLYTWDTLMPNINKLKTCGIHSKYMRAMYPTKTFPNHYTIVTGLYPESHGIIDNNMYDVNLNKNFSLSSEEQNNPAWWHGQPMWLTAMYQGLKAATYFWPGSEVAINGSFPSIYMPYNRSVPYEERISTLLKWLDLPKAERPSFYTMYFEEPDSSGHAGGPVSARVIKALQVVDHAFGMLMEGLKQRNLHNCVNIILLADHGMDQTYCNKMEYMTDYFPRINFYMYEGPAPRIRALNVPHDFFSFNSEEIVRNLSCRKPDQHFKPYLTPDLPKRLHYAKNVRIDKVHLFVDRQWLAVGSKSNTNCGGGNHGYNNEFRSMEAIFLAHGPSFKEKTEVEPFENIEVYNLMCDLLRIQPAPNNGTRGSLNHLLKVPFYEPSHAEEVSKFSVCGFANPLPTDNLSCLCPHLQNSIQLEQVNQMLNLTQEEITATVKVNLPFGRPRVLQKNVDNCLLYHREYVSGFGKAMRMPMWSSYTVPQLGDTSPLPPTVPDCLRADVRVPPSESQKCSFYLADENITHGFLYPPAINRTSDSQYDALIMSNLVPMYEEFRKMWDYFHSVLLIKHATERNGVNVVSGPIFDYNYDGHFDAPEEITKHIANTDIPIPTHYFVVLTSCKNKSHTPENCPGWLDVLPFIIPHRPTNVESCPEGKPEALWVEERFTAHIARVRDVELLTGLDFYQDKAQPVSEILQLKTYLPTFETTI
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Predicted Molecular Mass
97.2 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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