Enterovirus 71 VP0 Protein (Sf9, His)

The enterovirus 71 VP0 protein forms a 300 angstrom icosahedral capsid with the VP2 and VP3 proteins. Enterovirus 71 VP0 Protein (Sf9, His) is the recombinant virus-derived Enterovirus 71 VP0 protein, expressed by sf9 insect cells, with N-His labeled tag.

The Enterovirus 71 VP0 protein contributes to the formation of an icosahedral capsid with pseudo T=3 symmetry, alongside capsid proteins VP2 and VP3. This capsid, measuring 300 Angstroms in diameter, comprises 60 copies of each capsid protein and encapsulates the viral positive strand RNA genome. Capsid protein VP1 primarily shapes the vertices of the capsid, and, in conjunction with VP2, engages with the host cell receptor SCARB2 to facilitate virion attachment to target host cells, leading to clathrin-dependent endocytosis. Following receptor binding, the capsid undergoes conformational changes, resulting in the externalization of the N-terminus of capsid protein VP1, which contains an amphipathic alpha-helix, and capsid protein VP4. Together, these components create a pore in the host membrane, enabling the translocation of the viral genome into the host cell cytoplasm.

Virus

sf9 insect cells

N-His

Q66478 (M1-Q323)

/

Full Length of Capsid protein VP0

MGSQVSTQRSGSHENSNSATEGSTINYTTINYYKDSYAATAGKQSLKQDPDKFANPVKDIFTEMAAPLKSPSAEACGYSDRVAQLTIGNSTITTQEAANIIVGYGEWPSYCSDNDATAVDKPTRPDVSVNRFYTLDTKLWEKSSKGWYWKFPDVLTETGVFGPNAQFHYLYRSGFCIHVQCNASKFHQGALLVAVLPEYVIGTVAGGTGTENSHPPYKQTQPGADGFELQHPYVLDAGIPISQLTVCPHQWINLRTNNCATIIVPYMNTLPFDSALNHCNFGLLVVPISPLDFDQGATPVIPITITLAPMCSEFAGLRQAVTQ

65 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.