EPCR Protein, Cynomolgus (HEK293, His)

EPCR Protein plays a pivotal role in blood coagulation by binding to and enhancing the activation of protein C through interaction with the thrombin-thrombomodulin complex. Its significance lies in regulating coagulation processes and modulating protein C activation, a crucial factor in anticoagulant mechanisms. EPCR Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived EPCR protein, expressed by HEK293 , with C-His labeled tag. The total length of EPCR Protein, Cynomolgus (HEK293, His) is 192 a.a., with molecular weight of 30-47 kDa.

The EPCR protein exhibits a pivotal role in blood coagulation by binding to activated protein C and enhancing its activation through interaction with the thrombin-thrombomodulin complex. This participation in the protein C pathway underscores EPCR's significance in regulating coagulation processes, highlighting its ability to modulate the activation of protein C, a key factor in anticoagulant mechanisms.

Immobilized Human Activated Protein C at 3 µg/mL (100 µL/well) can bind Cynomolgus EPCR. The ED₅₀ for this effect is 2.076 μg/mL.

Cynomolgus

HEK293

C-6*His

XP_001100647.1 (S18-T209)

706040  [NCBI]

SQNASDGLQSLHMLQISYFRDPYHVWYQGNASLGGHLTHVLEGPGTNATILQLQPLQEPESWARMQSGLQAYLLEFHGLVRLVHQERTLAFPLTIRCFLGCELPPEGSRAHVFFEVAVNGSSFVSFRPETALWQADTQVPSKVVTFILQQLNAYNRTRYELREFLEDTCVQYVQKHISMENMKGSQTSRSYT

Approximately 30-47 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.