FBXL10 Protein, Human (sf9, FLAG)
FBXL10 Protein, Human (sf9, FLAG) is the recombinant human-derived FBXL10, expressed by Sf9 insect cells , with C-Flag labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
FBXL10 Protein, Human (sf9, FLAG) is the recombinant human-derived FBXL10, expressed by Sf9 insect cells , with C-Flag labeled tag.
Background
FBXL10 is a histone demethylase that specifically removes methylation modifications at 'Lys-4' and 'Lys-36' sites of histone H3, playing a central role in histone code regulation. This enzyme preferentially targets trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residues but exhibits weak or no activity toward mono- or trimethylated H3 'Lys-36'. FBXL10 primarily binds to the transcribed region of ribosomal RNA and represses ribosomal RNA gene transcription, thereby inhibiting cell growth and proliferation. Additionally, it may function as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag C-Flag
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Accession
Q8NHM5-1 (M1-Q650)
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Gene ID84678
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Molecular Construction
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N-term
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FBXL10 (M1-Q650)
Accession # Q8NHM5-1 -
FLAG
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C-term
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Protein Length
Partial
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Synonyms
KDM2B; Fbl10; Prev. FBXL10; Lysine (K)-Specific Demethylase 2B; F-Box And Leucine-Rich Repeat Protein 10; Protein-Containing CXXC Domain 2; JHDM1B; CXXC-Type Zinc Finger Protein 2; PCCX2; Jumonji Domain-Containing EMSY-Interactor Methyltransferase Motif P
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AA Sequence
MAGPQMGGSAEDHPPRKRHAAEKQKKKTVIYTKCFEFESATQRPIDRQRYDENEDLSDVEEIVSVRGFSLEEKLRSQLYQGDFVHAMEGKDFNYEYVQREALRVPLIFREKDGLGIKMPDPDFTVRDVKLLVGSRRLVDVMDVNTQKGTEMSMSQFVRYYETPEAQRDKLYNVISLEFSHTKLEHLVKRPTVVDLVDWVDNMWPQHLKEKQTEATNAIAEMKYPKVKKYCLMSVKGCFTDFHIDFGGTSVWYHVFRGGKIFWLIPPTLHNLALYEEWVLSGKQSDIFLGDRVERCQRIELKQGYTFFIPSGWIHAVYTPVDSLVFGGNILHSFNVPMQLRIYEIEDRTRVQPKFRYPFYYEMCWYVLERYVYCVTQRSHLTQEYQRESMLIDAPRKPSIDGFSSDSWLEMEEEACDQQPQEEEEKDEEGEGRDRAPKPPTDGSTSPTSTPSEDQEALGKKPKAPALRFLKRTLSNESEESVKSTTLAVDYPKTPTGSPATEVSAKWTHLTEFELKGLKALVEKLESLPENKKCVPEGIEDPQALLEGVKNVLKEHADDDPSLAITGVPVVTWPKKTPKNRAVGRPKGKLGPASAVKLAANRTTAGARRRRTRCRKCEACLRTECGECHFCKDMKKFGGPGRMKQSCIMRQ
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Predicted Molecular Mass
75 kDa
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Molecular Weight
Approximately 75-80 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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