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FCAR/CD89 Protein, Rat (HEK293, His)

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FCAR is an Fc fragment of the IgA receptor, also known as CD89. Fcars are found on the cell surface of myeloid cells, including neutrophils, monocytes, macrophages, and eosinophils. FCAR exerts proinflammatory effects by participating in PI3K and PLC-γ signaling. FCAR is also an important Fc receptor for neutrophils to kill tumor cells. FCAR/CD89 Protein, Rat (HEK293, His) is the recombinant rat-derived FCAR/CD89 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for FCAR/CD89 Protein, Rat (HEK293, His)
  • FCAR/CD89 Protein, Rat (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

FCAR is an Fc fragment of the IgA receptor, also known as CD89. Fcars are found on the cell surface of myeloid cells, including neutrophils, monocytes, macrophages, and eosinophils. FCAR exerts proinflammatory effects by participating in PI3K and PLC-γ signaling. FCAR is also an important Fc receptor for neutrophils to kill tumor cells. FCAR/CD89 Protein, Rat (HEK293, His) is the recombinant rat-derived FCAR/CD89 protein, expressed by HEK293 , with C-His labeled tag.

Background

The Fc fragment of the IgA receptor (FCAR) encodes the transmembrane receptor FC-α RI, also known as CD89. Fcars are found on the cell surface of myeloid cells, including neutrophils, monocytes, macrophages, and eosinophils. FCAR plays a role in both pro-inflammatory and anti-inflammatory responses, depending on its binding status to IgA. FCAR exerts proinflammatory effects by participating in PI3K and PLC-γ signaling. FCAR is also an important Fc receptor for neutrophils to kill tumor cells[1][2].

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat FCAR at 1 µg/mL can bind Rat IgA with an apparent KD is 5.683 nM.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-His
  • Accession

    D3ZRL6/NP_973721.1 (Q22-N228)

  • Gene ID
    365183  [NCBI]
  • Molecular Construction
    • N-term
    • FCAR (Q22-N228)
      Accession # D3ZRL6/NP_973721.1
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    FCAR; Immunoglobulin Alpha Fc Receptor; Fc Alpha Receptor; Fc Fragment Of IgA Receptor; CD89; IgA Fc Receptor; FcalphaRI; FCAR Variant 14; FcalphaR; CD89 Antigen; Fc Fragment Of IgA, Receptor For; CTB-61M7.2

  • AA Sequence

    QEDFPIPIISTATNPMVSWNESVRILCRGTPEAFLYQLSLMKNSTQTVIEKKLGFQKEAEFIINHMNSTLAGCYQCQYRKKNHWSEQSKPLKLVVTGLYDKPVLSTDQSLVLLPGENVSFKCSSAHNLFNRFSLAKEGEASLPLHQHKESQGNFHLGPVNADFIGKYRCYGWHNNSPYVWSAPSDALELIVSVTSLDSEKHDYVMEN

  • Molecular Weight

    Approximately 35-40 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • FCAR/CD89 Protein, Rat (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for FCAR/CD89 Protein, Rat (HEK293, His)
  • FCAR/CD89 Protein, Rat (HEK293, His) Product Image