FLRT2 Protein, Human (HEK293, His)

FLRT2 protein is involved in cell-cell adhesion, migration, and axonal guidance through various interactions. It promotes adhesion by binding to ADGRL3 and potentially other sloth proteins. FLRT2 Protein, Human (HEK293, His) is the recombinant human-derived FLRT2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of FLRT2 Protein, Human (HEK293, His) is 504 a.a., with molecular weight of 75-85 kDa.

The FLRT2 protein operates in cell-cell adhesion, cell migration, and axon guidance, participating in these processes through various molecular interactions. It facilitates cell-cell adhesion by engaging with ADGRL3 and potentially other latrophilins present on the surface of neighboring cells. In brain development, FLRT2 may contribute to the migration of cortical neurons by interacting with UNC5D. Additionally, it plays a role in axon growth cone collapse and functions as a repulsive cue in neuron guidance, particularly through its interaction with UNC5D and potentially other UNC-5 family members. FLRT2 is implicated in fibroblast growth factor-mediated signaling cascades. It is essential for the proper organization of the cardiac basement membrane during embryogenesis and contributes to normal embryonic epicardium and heart morphogenesis. The protein self-associates via leucine-rich repeats, forming homooligomers. FLRT2 interacts with FGFR1 and FGFR2, as well as with ADGRL1/LPHN1, ADGRL3, UNC5D, UNC5B (with lower affinity), and FN1 through its extracellular domain, highlighting its diverse roles in cellular processes and developmental events.

Human

HEK293

C-6*His

O43155 (C36-S539)

23768  [NCBI]

CPSVCRCDRNFVYCNERSLTSVPLGIPEGVTVLYLHNNQINNAGFPAELHNVQSVHTVYLYGNQLDEFPMNLPKNVRVLHLQENNIQTISRAALAQLLKLEELHLDDNSISTVGVEDGAFREAISLKLLFLSKNHLSSVPVGLPVDLQELRVDENRIAVISDMAFQNLTSLERLIVDGNLLTNKGIAEGTFSHLTKLKEFSIVRNSLSHPPPDLPGTHLIRLYLQDNQINHIPLTAFSNLRKLERLDISNNQLRMLTQGVFDNLSNLKQLTARNNPWFCDCSIKWVTEWLKYIPSSLNVRGFMCQGPEQVRGMAVRELNMNLLSCPTTTPGLPLFTPAPSTASPTTQPPTLSIPNPSRSYTPPTPTTSKLPTIPDWDGRERVTPPISERIQLSIHFVNDTSIQVSWLSLFTVMAYKLTWVKMGHSLVGGIVQERIVSGEKQHLSLVNLEPRSTYRICLVPLDAFNYRAVEDTICSEATTHASYLNNGSNTASSHEQTTSHSMGS

75-85 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.