FSTL3 Protein, Human (HEK293, His)
Based on 1 publication(s) in Google Scholar
FSTL3 proteins, specifically isoform 1, act as secretory antagonists of TGF-β family members such as activin, BMP2, and MSTN. It can effectively inhibit cell signaling induced by activin A, activin B, BMP2 and MSDT, and has a stronger effect on activin A. FSTL3 Protein, Human (HEK293, His) is the recombinant human-derived FSTL3 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
FSTL3 proteins, specifically isoform 1, act as secretory antagonists of TGF-β family members such as activin, BMP2, and MSTN. It can effectively inhibit cell signaling induced by activin A, activin B, BMP2 and MSDT, and has a stronger effect on activin A. FSTL3 Protein, Human (HEK293, His) is the recombinant human-derived FSTL3 protein, expressed by HEK293 , with C-His labeled tag.
Background
FSTL3, specifically Isoform 1, functions as a secreted binding and antagonizing protein for various members of the TGF-beta family, including activin, BMP2, and MSTN. It effectively inhibits cellular signaling induced by activin A, activin B, BMP2, and MSDT, displaying greater efficacy against activin A than activin B. FSTL3 plays a vital role in bone formation by inhibiting osteoclast differentiation. Additionally, it contributes to hematopoiesis, influencing the differentiation of hemopoietic progenitor cells and promoting their adhesion to fibronectin, thereby facilitating their interaction with the bone marrow stroma. On the other hand, Isoform 2, or the nuclear form, is likely involved in transcriptional regulation through its interaction with MLLT10. FSTL3 interacts with various proteins, including INHBA, INHBB, FN1, ADAM12, and MSTN, highlighting its versatility in mediating diverse cellular processes and molecular interactions. Notably, the interaction between Isoform 2 and MLLT10 enhances both in vitro transcriptional activity and self-association of MLLT10.
Verified Bioactivity
1.Immobilized Human FSTL3, His Tag at 0.5 μg/mL (100 μl/Well) on the plate. Dose response curve for Anti-FSTL3 Antibody, hFc Tag with the EC50 of ≤27.5 ng/mL determined by ELISA.
2.Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 5-25 ng/mL in the presence of 7.5 ng/mL rhActivin A.
3.Immobilized Human Activin A, No Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Human FSTL3, His Tag with the EC50 of 10.6 ng/mL determined by ELISA.
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
Cell Death Dis
Cancer-associated fibroblasts expressing FSTL3 promote vasculogenic mimicry formation and drive colon cancer malignancy. [Abstract]2025 Oct 6;16(1):706. PMID: 41053124
Technical Parameters
-
Species Human
-
Source HEK293
-
Tag C-8*His
-
Accession
O95633 (M27-V263)
-
Molecular Construction
-
N-term
-
FSTL3 (M27-V263)
Accession # O95633 -
8*His
-
C-term
-
-
Protein Length
Full Length of Mature Protein
-
Synonyms
FSTL3; Follistatin-Related Gene Protein; Follistatin Like 3; Follistatin-Related Protein 3; FLRG; Follistatin-Like Protein 3; FSRP; Follistatin-Related Protein; Follistatin-Like 3 (Secreted Glycoprotein)
-
AA Sequence
MGSGNPAPGGVCWLQQGQEATCSLVLQTDVTRAECCASGNIDTAWSNLTHPGNKINLLGFLGLVHCLPCKDSCDGVECGPGKACRMLGGRPRCECAPDCSGLPARLQVCGSDGATYRDECELRAARCRGHPDLSVMYRGRCRKSCEHVVCPRPQSCVVDQTGSAHCVVCRAAPCPVPSSPGQELCGNNNVTYISSCHMRQATCFLGRSIGVRHAGSCAGTPEEPPGGESAEEEENFV
-
Predicted Molecular Mass
26.1 kDa
-
Molecular Weight
Approximately 35-40 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
-
Glycosylation
Yes
-
Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (237 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
