FTO Protein, Human (His)
Based on 1 Customer Validation
FTO protein is an RNA demethylase that regulates fat mass, lipogenesis, and energy homeostasis by targeting N(6)-methyladenosine in mRNA. It also demethylates m6A in U6 snRNA and m6A(m) in 5'-cap, affecting mRNA stability. FTO Protein, Human (His) is the recombinant human-derived FTO protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Almacenamiento:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Technical Parameters
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Product Properties
- Documentación
- Help & FAQs
Actividad biológica
Descripciòn
FTO protein is an RNA demethylase that regulates fat mass, lipogenesis, and energy homeostasis by targeting N(6)-methyladenosine in mRNA. It also demethylates m6A in U6 snRNA and m6A(m) in 5'-cap, affecting mRNA stability. FTO Protein, Human (His) is the recombinant human-derived FTO protein, expressed by E. coli , with N-6*His labeled tag.
Background
The FTO protein functions as an RNA demethylase, mediating oxidative demethylation across various RNA species, including mRNAs, tRNAs, and snRNAs. It plays a crucial role in regulating fat mass, adipogenesis, and energy homeostasis. Specifically, FTO targets the most prevalent internal modification of mRNA, N(6)-methyladenosine (m6A), affecting mRNA expression and stability. Additionally, FTO can demethylate m6A in U6 small nuclear RNA (snRNA) and N(6),2'-O-dimethyladenosine cap (m6A(m)). The demethylation of m6A(m) in the 5'-cap by FTO influences mRNA stability by promoting susceptibility to decapping. Acting as a tRNA demethylase, FTO removes N(1)-methyladenine from various tRNAs. Beyond its role in RNA modification, FTO contributes to the regulation of fat mass, adipogenesis, body weight, and thermogenesis. It also plays a significant role in the regulation of dopaminergic midbrain circuitry and acts as an oncogenic factor in certain acute myeloid leukemias by enhancing the expression of target transcripts such as MYC, CEBPA, ASB2, and RARA through m6A demethylation.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
Q9C0B1 (T32-P505)
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Gene ID79068
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Molecular Construction
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N-term
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6*His
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FTO (T32-P505)
Accession # Q9C0B1 -
C-term
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Protein Length
Partial
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Synonyms
FTO; IFEX9; FTO Alpha-Ketoglutarate Dependent Dioxygenase; Intragenic FTO Exon 9 Containing Transcript; U6 Small Nuclear RNA (2'-O-Methyladenosine-N(6)-)-Demethylase FTO; MRNA N(6)-Methyladenine Demethylase; U6 Small Nuclear RNA N(6)-Methyladenosine-Demethylase FTO; Fat Mass And Obesity Associated; MRNA (2'-O-Methyladenosine-N(6)-)-Demethylase FTO; M6A(M)-Demethylase FTO; Alpha-Ketoglutarate-Dependent Dioxygenase FTO; AlkB Homolog 9; MRNA N(6)-Methyladenosine Demethylase FTO; MGC5149; Fat Mass And Obesity-Associated Protein; FTO, Alpha-Ketoglutarate Dependent Dioxygenase; TRNA N1-Methyl Adenine Demethylase FTO; Alpha-Ketoglutarate-Dependent Dioxygenase; KIAA1752; BMIQ14; ALKBH9; GDFD
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AA Sequence
TPKDDEFYQQWQLKYPKLILREASSVSEELHKEVQEAFLTLHKHGCLFRDLVRIQGKDLLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSNIKHTEAEIAAACETFLKLNDYLQIETIQALEELAAKEKANEDAVPLCMSADFPRVGMGSSYNGQDEVDIKSRAAYNVTLLNFMDPQKMPYLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYSYSCEGPEEESEDDSHLEGRDPDIWHVGFKISWDIETPGLAIPLHQGDCYFMLDDLNATHQHCVLAGSQPRFSSTHRVAECSTGTLDYILQRCQLALQNVCDDVDNDDVSLKSFEPAVLKQGEEIHNEVEFEWLRQFWFQGNRYRKCTDWWCQPMAQLEALWKKMEGVTNAVLHEVKREGLPVEQRNEILTAILASLTARQNLRREWHARCQSRIARTLPADQKPECRPYWEKDDASMPLPFDLTDIVSELRGQLLEAKP
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Predicted Molecular Mass
83.2 kDa
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Peso molecular
Approximately 85 kDa, based on SDS-PAGE under reducing conditions.
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Pureza
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentación
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Ficha de datos (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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