FUCA1 Protein, Human (HEK293, His)
Based on 1 Customer Validation
FUCA1 Proteinas, as alpha-L-fucosidase, plays a pivotal role in hydrolyzing alpha-1,6-linked fucose residues on glycoproteins' carbohydrate moieties. FUCA1 Protein, Human (HEK293, His) is the recombinant human-derived FUCA1 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
FUCA1 Proteinas, as alpha-L-fucosidase, plays a pivotal role in hydrolyzing alpha-1,6-linked fucose residues on glycoproteins' carbohydrate moieties. FUCA1 Protein, Human (HEK293, His) is the recombinant human-derived FUCA1 protein, expressed by HEK293 , with C-His labeled tag.
Background
FUCA1 protein serves a pivotal role as alpha-L-fucosidase, responsible for the hydrolysis of the alpha-1,6-linked fucose residue connected to the reducing-end N-acetylglucosamine within the carbohydrate moieties of glycoproteins.
Verified Bioactivity
Measured by its ability to cleave a fluorogenic substrate 4-Methylumbelliferyl-alpha-L-fucopyranoside. The specific activity is >780 pmol/min/µg, as measured under the described conditions.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
P04066 (Q32-K466)
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Molecular Construction
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N-term
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FUCA1 (Q32-K466)
Accession # P04066 -
His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
FUCA1; Tissue Fucosidase; Alpha-L-Fucosidase 1; A-L-Fucosidase 1; Alpha-L-Fucoside Fucohydrolase 1; I+/--L-Fucosidase 1; Fucosidase, Alpha-L- 1, Tissue; Α-L-Fucosidase 1; Tissue Alpha-L-Fucosidase; FUCA; Alpha-L-Fucosidase I
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AA Sequence
QPPRRYTPDWPSLDSRPLPAWFDEAKFGVFIHWGVFSVPAWGSEWFWWHWQGEGRPQYQRFMRDNYPPGFSYADFGPQFTARFFHPEEWADLFQAAGAKYVVLTTKHHEGFTNWPSPVSWNWNSKDVGPHRDLVGELGTALRKRNIRYGLYHSLLEWFHPLYLLDKKNGFKTQHFVSAKTMPELYDLVNSYKPDLIWSDGEWECPDTYWNSTNFLSWLYNDSPVKDEVVVNDRWGQNCSCHHGGYYNCEDKFKPQSLPDHKWEMCTSIDKFSWGYRRDMALSDVTEESEIISELVQTVSLGGNYLLNIGPTKDGLIVPIFQERLLAVGKWLSINGEAIYASKPWRVQWEKNTTSVWYTSKGSAVYAIFLHWPENGVLNLESPITTSTTKITMLGIQGDLKWSTDPDKGLFISLPQLPPSAVPAEFAWTIKLTGVK
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Molecular Weight
Approximately 57 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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