1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Isomerases (EC 5)
  4. FUOM/Fucose Mutarotase Protein, Human (His)

FUOM/Fucose Mutarotase Protein, Human (His)

Cat. No.: HY-P76940
SDS COA Handling Instructions

The FUOM/fucose mutarotase protein has a crucial interconversion effect on α- and β-L-fucose, with β-L-fucose being dominant (70.5%). The β-form undergoes metabolic processes via the salvage pathway, producing GDP-L-fucose that is transported to the endoplasmic reticulum. FUOM/Fucose Mutarotase Protein, Human (His) is the recombinant human-derived FUOM/Fucose Mutarotase protein, expressed by E. coli , with N-His labeled tag.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

The FUOM/fucose mutarotase protein has a crucial interconversion effect on α- and β-L-fucose, with β-L-fucose being dominant (70.5%). The β-form undergoes metabolic processes via the salvage pathway, producing GDP-L-fucose that is transported to the endoplasmic reticulum. FUOM/Fucose Mutarotase Protein, Human (His) is the recombinant human-derived FUOM/Fucose Mutarotase protein, expressed by E. coli , with N-His labeled tag.

Background

The FUOM/Fucose Mutarotase Protein plays a crucial role in the interconversion between alpha- and beta-L-fucoses, where L-fucose, a 6-deoxy-L-galactose, coexists as alpha-L-fucose (29.5%) and beta-L-fucose (70.5%). Notably, the beta-form undergoes metabolic processes through the salvage pathway. GDP-L-fucose, generated via either the de novo or salvage pathways, is transported to the endoplasmic reticulum. There, it serves as a substrate for N- and O-glycosylations catalyzed by fucosyltransferases. Fucosylated structures expressed on cell surfaces or secreted in biological fluids are thought to play a pivotal role in cell-cell adhesion and recognition processes. The dynamic interconversion facilitated by FUOM highlights its significance in modulating the pool of available L-fucose, influencing essential glycosylation events crucial for various cellular functions.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

E. coli

Tag

N-His

Accession

A2VDF0-1 (M1-L154)

Gene ID
Molecular Construction
N-term
His
FUOM (M1-L154)
Accession # A2VDF0-1
C-term
Synonyms
Fucose Mutarotase; FUOM; C10orf125
Molecular Weight

Approximately 19 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

FUOM/Fucose Mutarotase Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
FUOM/Fucose Mutarotase Protein, Human (His)
Cat. No.:
HY-P76940
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