GAD1/GAD67 Protein, Human (sf9, His)

The GAD1/GAD67 protein utilizes pyridoxal 5'-phosphate as a cofactor and acts as a catalyst in the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). GAD1 plays a key role in the conversion of glutamate to GABA, a key neurotransmitter involved in regulating neuronal excitability. GAD1/GAD67 Protein, Human (sf9, His) is the recombinant human-derived GAD1/GAD67 protein, expressed by Sf9 insect cells , with C-His labeled tag.

Glutamate Decarboxylase 1 (GAD1), also known as GAD67, is an enzyme that plays a pivotal role in neurotransmission by catalyzing the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). GAD1 utilizes pyridoxal 5'-phosphate as a cofactor in the decarboxylation of glutamate to form GABA, a crucial neurotransmitter that regulates neuronal excitability in the central nervous system. The balanced production of GABA by GAD1 is essential for maintaining the inhibitory-excitatory balance in neural circuits and is implicated in various physiological and pathological conditions of the nervous system.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

C-10*His

Q99259-1 (M1-L594)

2571  [NCBI]

Full Length of Isoform-1

MASSTPSSSATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFKERQSSKNLLSCENSDRDARFRRTETDFSNLFARDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMFTYEIAPVFVLMEQITLKKMREIVGWSSKDGDGIFSPGGAISNMYSIMAARYKYFPEVKTKGMAAVPKLVLFTSEQSHYSIKKAGAALGFGTDNVILIKCNERGKIIPADFEAKILEAKQKGYVPFYVNATAGTTVYGAFDPIQEIADICEKYNLWLHVDAAWGGGLLMSRKHRHKLNGIERANSVTWNPHKMMGVLLQCSAILVKEKGILQGCNQMCAGYLFQPDKQYDVSYDTGDKAIQCGRHVDIFKFWLMWKAKGTVGFENQINKCLELAEYLYAKIKNREEFEMVFNGEPEHTNVCFWYIPQSLRGVPDSPQRREKLHKVAPKIKALMMESGTTMVGYQPQGDKANFFRMVISNPAATQSDIDFLIEEIERLGQDL

Approximately 68-74 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

• 
  ≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.