Galectin-14/LGALS14 Protein, Human (HEK293, His)
Based on 1 Customer Validation
PPL13/LGALS14 is a mammalian placenta-specific galectin with placental specificity. Many placental lectins induce apoptosis of activated T cells and other leukocytes, thereby conferring immune tolerance to the recipient. Galectin-14/LGALS14 Protein, Human (HEK293, His) is the recombinant human-derived Galectin-14/LGALS14 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
PPL13/LGALS14 is a mammalian placenta-specific galectin with placental specificity. Many placental lectins induce apoptosis of activated T cells and other leukocytes, thereby conferring immune tolerance to the recipient. Galectin-14/LGALS14 Protein, Human (HEK293, His) is the recombinant human-derived Galectin-14/LGALS14 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
PPL13/LGALS14 belongs to the placenta-specific protein family and are proteins secreted by the placenta into the maternal circulation. Human placenta-specific galectin is primarily expressed by the syncytiotrophoblast, the primary site of metabolic exchange. Early in pregnancy, the fetus comes into contact with immune cells circulating in the maternal blood. In ex vivo functional assays, placenta-specific galectin was found to induce T lymphocyte apoptosis, so galectin may reduce the risk of maternal immune attack on fetal semi-allografts and may confer additional immune tolerance. Mechanisms that maintain the hemorrhagic placenta during long gestations in humanoid primates. However, these proteins are reduced along with abnormal placenta before the onset of intrauterine growth restriction (IUGR) or preeclampsia (PE). PPL13 is highly expressed in pregnancies complicated by PE and IUGR. The PPL13 protein binds β-galactoside and lactose and is a strong inducer of T cell apoptosis.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
AAH22257.1 (M1-D139)
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Molecular Construction
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N-term
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LGALS14 (M1-D139)
Accession # AAH22257.1 -
6*His
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C-term
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Protein Length
Full Length
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Synonyms
LGALS14; Placental Protein 13-Like; Galectin 14; Gal-14; PPL13; Placental Protein 13-Like Protein; CLC2; Galectin-14; Lectin, Galactoside-Binding, Soluble, 14; Galectin; Charcot-Leyden Crystal Protein 2
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AA Sequence
MSSLPVPYTLPVSLPVGSCVIITGTPILTFVKDPQLEVNFYTGMDEDSDIAFQFRLHFGHPAIMNSRVFGIWRYEEKCYYLPFEDGKPFELCIYVRHKEYKVMVNGQRIYNFAHRFPPASVKMLQVLRDISLTRVLISD
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Predicted Molecular Mass
17.1 kDa
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Molecular Weight
Approximately 16 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 20% glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (236 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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