Gamma-hemolysin component A Protein, S. aureus (Myc, His)
Gamma-hemolysin component A (HLgA) functions as a toxin and creates cell membrane pores with hemolytic and leukotoxic activities. Its action depends on sequential binding and cooperative association with class S and class F components to form heterooligomeric complexes. Gamma-hemolysin component A Protein, S. aureus (Myc, His) is the recombinant Staphylococcus aureus-derived Gamma-hemolysin component A protein, expressed by E. coli , with N-His, C-Myc labeled tag.
- Species: Staphylococcus aureus
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
Gamma-hemolysin component A (HLgA) functions as a toxin and creates cell membrane pores with hemolytic and leukotoxic activities. Its action depends on sequential binding and cooperative association with class S and class F components to form heterooligomeric complexes. Gamma-hemolysin component A Protein, S. aureus (Myc, His) is the recombinant Staphylococcus aureus-derived Gamma-hemolysin component A protein, expressed by E. coli , with N-His, C-Myc labeled tag.
Background
Gamma-hemolysin component A (HlgA) is a toxin that operates by creating pores in the cell membrane, exhibiting both hemolytic and leucotoxic activities. Its toxic effects are contingent upon the sequential binding and synergistic association of a class S and a class F component, resulting in the formation of heterooligomeric complexes. In strains producing both gamma-hemolysins and leukocidins, HlgA (class S) associates with HlgB (class F), thus constituting an AB toxin. Moreover, HlgA can also form a complex with LukF-PV, showcasing the versatile interactions and potentially cooperative mechanisms employed by this toxin.
Technical Parameters
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Species Staphylococcus aureus
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Source E. coli
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Tag N-His;C-Myc
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Accession
P0A073 (E30-K309)
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Gene ID59701246
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Molecular Construction
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N-term
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10*His
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hlgA (E30-K309)
Accession # P0A073 -
Myc
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
GLS2; Phosphate-Activated Glutaminase; Glutaminase 2; Phosphate-Dependent Glutaminase; GLS; Alternative Protein GLS2; GA; Breast Cell Glutaminase; L-Glutamine Amidohydrolase; Truncated Glutaminase 2; HLGA; Glutaminase I; LGA; L-Glutaminase; Glutaminase Li
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AA Sequence
ENKIEDIGQGAEIIKRTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMIWPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNYSKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARDYFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAVDRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK
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Predicted Molecular Mass
39.4 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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