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  4. Gamma-hemolysin component A Protein, S. aureus (Myc, His)

Gamma-hemolysin component A Protein, S. aureus (Myc, His)

Cat. No.: HY-P71485
Handling Instructions

Gamma-hemolysin component A (HLgA) functions as a toxin and creates cell membrane pores with hemolytic and leukotoxic activities. Its action depends on sequential binding and cooperative association with class S and class F components to form heterooligomeric complexes. Gamma-hemolysin component A Protein, S. aureus (Myc, His) is the recombinant Staphylococcus aureus-derived Gamma-hemolysin component A protein, expressed by E. coli , with N-His, C-Myc labeled tag. The total length of Gamma-hemolysin component A Protein, S. aureus (Myc, His) is 280 a.a., with molecular weight of ~39.4 kDa.

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Description

Gamma-hemolysin component A (HLgA) functions as a toxin and creates cell membrane pores with hemolytic and leukotoxic activities. Its action depends on sequential binding and cooperative association with class S and class F components to form heterooligomeric complexes. Gamma-hemolysin component A Protein, S. aureus (Myc, His) is the recombinant Staphylococcus aureus-derived Gamma-hemolysin component A protein, expressed by E. coli , with N-His, C-Myc labeled tag. The total length of Gamma-hemolysin component A Protein, S. aureus (Myc, His) is 280 a.a., with molecular weight of ~39.4 kDa.

Background

Gamma-hemolysin component A (HlgA) is a toxin that operates by creating pores in the cell membrane, exhibiting both hemolytic and leucotoxic activities. Its toxic effects are contingent upon the sequential binding and synergistic association of a class S and a class F component, resulting in the formation of heterooligomeric complexes. In strains producing both gamma-hemolysins and leukocidins, HlgA (class S) associates with HlgB (class F), thus constituting an AB toxin. Moreover, HlgA can also form a complex with LukF-PV, showcasing the versatile interactions and potentially cooperative mechanisms employed by this toxin.

Species

Staphylococcus aureus

Source

E. coli

Tag

N-His;C-Myc

Accession

P0A073 (30E-309K)

Gene ID

59701246

Molecular Construction
N-term
10*His
hlgA (30E-309K)
Accession # P0A073
Myc
C-term
Synonyms
hlgA; hlg2; MW2342; Gamma-hemolysin component A; H-gamma-2; H-gamma-II
AA Sequence

ENKIEDIGQGAEIIKRTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMIWPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNYSKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARDYFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAVDRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK

Molecular Weight

Approximately 39.4 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Gamma-hemolysin component A Protein, S. aureus (Myc, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Gamma-hemolysin component A Protein, S. aureus (Myc, His)
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HY-P71485
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