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  4. Gamma-hemolysin component C Protein, S. aureus (P.pastoris, His)

Gamma-hemolysin component C Protein, S. aureus (P.pastoris, His)

Cat. No.: HY-P71835
Handling Instructions

Gamma-hemolysin component C (HLgC) acts as a toxin, forming cell membrane pores with hemolytic and leukotoxic activities. The toxicity of HlgC requires sequential binding and cooperative association with S- and F-class components to form heterooligomeric complexes. Gamma-hemolysin component C Protein, S. aureus (P.pastoris, His) is the recombinant Staphylococcus aureus-derived Gamma-hemolysin component C protein, expressed by P. pastoris , with N-His labeled tag. The total length of Gamma-hemolysin component C Protein, S. aureus (P.pastoris, His) is 286 a.a., with molecular weight of ~34.6 kDa.

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Description

Gamma-hemolysin component C (HLgC) acts as a toxin, forming cell membrane pores with hemolytic and leukotoxic activities. The toxicity of HlgC requires sequential binding and cooperative association with S- and F-class components to form heterooligomeric complexes. Gamma-hemolysin component C Protein, S. aureus (P.pastoris, His) is the recombinant Staphylococcus aureus-derived Gamma-hemolysin component C protein, expressed by P. pastoris , with N-His labeled tag. The total length of Gamma-hemolysin component C Protein, S. aureus (P.pastoris, His) is 286 a.a., with molecular weight of ~34.6 kDa.

Background

Gamma-hemolysin component C (HlgC) is a toxin known for its mechanism of action involving the formation of pores in the cell membrane, resulting in both hemolytic and leucotoxic activities. The toxicity of HlgC necessitates a sequential binding and synergistic association of a class S and a class F component, giving rise to the formation of heterooligomeric complexes. In particular, HlgC, belonging to the class S, associates with HlgB, a class F component, forming a CB toxin. This cooperative interaction underscores the intricate molecular processes employed by HlgC to exert its toxic effects on target cells.

Species

Staphylococcus aureus

Source

P. pastoris

Tag

N-His

Accession

Q7A3S2 (30A-315N)

Gene ID

/

Molecular Construction
N-term
His
hlgC (30A-315N)
Accession # Q7A3S2
C-term
Synonyms
hlgC; SA2208Gamma-hemolysin component C
AA Sequence

ANDTEDIGKGSDIEIIKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKAMRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSFNYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKDPRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN

Molecular Weight

Approximately 34.6 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Gamma-hemolysin component C Protein, S. aureus (P.pastoris, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Gamma-hemolysin component C Protein, S. aureus (P.pastoris, His)
Cat. No.:
HY-P71835
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