Gamma-hemolysin component C Protein, S. aureus (P.pastoris, His)
Gamma-hemolysin component C (HLgC) acts as a toxin, forming cell membrane pores with hemolytic and leukotoxic activities. The toxicity of HlgC requires sequential binding and cooperative association with S- and F-class components to form heterooligomeric complexes. Gamma-hemolysin component C Protein, S. aureus (P.pastoris, His) is the recombinant Staphylococcus aureus-derived Gamma-hemolysin component C protein, expressed by P. pastoris , with N-His labeled tag.
- Species: Staphylococcus aureus
- Source: P. pastoris
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
Gamma-hemolysin component C (HLgC) acts as a toxin, forming cell membrane pores with hemolytic and leukotoxic activities. The toxicity of HlgC requires sequential binding and cooperative association with S- and F-class components to form heterooligomeric complexes. Gamma-hemolysin component C Protein, S. aureus (P.pastoris, His) is the recombinant Staphylococcus aureus-derived Gamma-hemolysin component C protein, expressed by P. pastoris , with N-His labeled tag.
Background
Gamma-hemolysin component C (HlgC) is a toxin known for its mechanism of action involving the formation of pores in the cell membrane, resulting in both hemolytic and leucotoxic activities. The toxicity of HlgC necessitates a sequential binding and synergistic association of a class S and a class F component, giving rise to the formation of heterooligomeric complexes. In particular, HlgC, belonging to the class S, associates with HlgB, a class F component, forming a CB toxin. This cooperative interaction underscores the intricate molecular processes employed by HlgC to exert its toxic effects on target cells.
Technical Parameters
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Species Staphylococcus aureus
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Source P. pastoris
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Tag N-His
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Accession
Q7A3S2 (A30-N315)
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Gene ID/
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Molecular Construction
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N-term
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His
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hlgC (A30-N315)
Accession # Q7A3S2 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
Gamma-hemolysin component C; hlgC; SA2208
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AA Sequence
ANDTEDIGKGSDIEIIKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKAMRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSFNYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKDPRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN
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Predicted Molecular Mass
34.6 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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