GFAP Protein, Mouse (His)
Based on 1 Customer Validation
The GFAP protein is a member of the class III intermediate filament family and serves as a cell-specific marker critical for distinguishing astrocytes from other glial cells in the developing central nervous system. Its role as a marker is critical in defining the identity of astrocytes, highlighting their unique characteristics in a complex cellular environment. GFAP Protein, Mouse (His) is the recombinant mouse-derived GFAP protein, expressed by E. coli, with N-His labeled tag.
- Species: Mouse
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The GFAP protein is a member of the class III intermediate filament family and serves as a cell-specific marker critical for distinguishing astrocytes from other glial cells in the developing central nervous system. Its role as a marker is critical in defining the identity of astrocytes, highlighting their unique characteristics in a complex cellular environment. GFAP Protein, Mouse (His) is the recombinant mouse-derived GFAP protein, expressed by E. coli, with N-His labeled tag.
Glial Fibrillary Acidic Protein (GFAP), classified as a class-III intermediate filament, functions as a cell-specific marker crucial for distinguishing astrocytes from other glial cells, particularly during the development of the central nervous system. GFAP plays a pivotal role in the formation and maintenance of the cytoskeleton in astrocytes, contributing to their structural support and stability. Its expression is highly specific to astrocytes, making it a valuable marker in identifying these glial cells in various physiological and pathological contexts. Furthermore, GFAP has been reported to interact with SYNM, suggesting potential functional associations with proteins involved in cytoskeletal dynamics or cellular signaling pathways within astrocytes. This interaction adds a layer of complexity to the understanding of GFAP's role in astrocyte biology. Ongoing research may reveal additional details about the molecular mechanisms and functional implications of GFAP in the central nervous system.
Technical Parameters
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Species Mouse
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Source E. coli
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Tag N-His
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Accession
P03995 (M1-M430)
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Gene ID14580
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Molecular Construction
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N-term
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His
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GFAP (M1-M430)
Accession # P03995 -
C-term
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Protein Length
Full Length
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Synonyms
GFAP; Intermediate Filament Protein; Glial Fibrillary Acidic Protein; ALXDRD; FLJ45472
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AA Sequence
MERRRITSARRSYASETVVRGLGPSRQLGTMPRFSLSRMTPPLPARVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNFAQDLGTLRQKLQDETNLRLEAENNLAAYRQEADEATLARVDLERKVESLEEEIQFLRKIYEEEVRELREQLAQQQVHVEMDVAKPDLTAALREIRTQYEAVATSNMQETEEWYRSKFADLTDAASRNAELLRQAKHEANDYRRQLQALTCDLESLRGTNESLERQMREQEERHARESASYQEALARLEEEGQSLKEEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKDSKQEHKDVVM
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Predicted Molecular Mass
51.9 kDa
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Molecular Weight
Approximately 55-60 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from 0.22 μm filtered solution of 20 mM Tris, 0.5 M Arginine, 150 mM NaCl, pH 8.0 with 10% trehalose as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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