GltI/YbeJ Protein, E.coli (His-SUMO)
The GltI/YbeJ protein is a key component of the ABC transporter complex GltIJKL and is essential for active transport of glutamate and aspartate. As the solute-binding protein in the complex, GltI binds glutamate and aspartate. GltI/YbeJ Protein, E.coli (His-SUMO) is the recombinant E. coli-derived GltI/YbeJ protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
- Species: E.coli
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The GltI/YbeJ protein is a key component of the ABC transporter complex GltIJKL and is essential for active transport of glutamate and aspartate. As the solute-binding protein in the complex, GltI binds glutamate and aspartate. GltI/YbeJ Protein, E.coli (His-SUMO) is the recombinant E. coli-derived GltI/YbeJ protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
Background
GltI/YbeJ protein is an integral component of the ABC transporter complex GltIJKL, crucial for the active uptake of glutamate and aspartate. Operating as a solute-binding protein within the complex, GltI plays a pivotal role in binding both glutamate and aspartate. This complex comprises two ATP-binding proteins (GltL), two transmembrane proteins (GltJ and GltK), and the solute-binding protein GltI. Together, these components orchestrate the efficient transport of glutamate and aspartate across the cell membrane, highlighting the essential function of GltI/YbeJ in this process.
Technical Parameters
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Species E.coli
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Source E. coli
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Tag N-His;N-SUMO
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Accession
P37902 (D23-N302)
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Gene ID946938 [NCBI]
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Molecular Construction
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N-term
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6*His-SUMO
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GltI (D23-N302)
Accession # P37902 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
Glutamate/aspartate import solute-binding protein; gltI; ybeJ; yzzK; b0655; JW5092
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AA Sequence
DDAAPAAGSTLDKIAKNGVIVVGHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLLQNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFANLKDKAVVVTSGTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKKPDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPPKNLNMNFELSDEMKALFKEPNDKALN
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Predicted Molecular Mass
47.2 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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