GMP IL-18 Protein, Human (HEK293, His)

IL-18 protein is a pro-inflammatory cytokine that plays a critical role in epithelial barrier repair and polarized immune responses involving Th1 cells and NK cells. After binding to IL18R1 and IL18RAP, it forms a signaling ternary complex that activates NF-kappa-B, leading to the synthesis of inflammatory mediators. GMP IL-18 Protein, Human (HEK293, His) is the recombinant human-derived IL-18 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of GMP IL-18 Protein, Human (HEK293, His) is 157 a.a., with molecular weight of ~17 kDa.

IL-18 Protein is a pro-inflammatory cytokine with pivotal roles in epithelial barrier repair and orchestrating polarized immune responses involving T-helper 1 (Th1) cells and natural killer (NK) cells. Upon binding to IL18R1 and IL18RAP, IL-18 forms a signaling ternary complex that activates NF-kappa-B, leading to the synthesis of inflammatory mediators. It synergizes with IL-12 to induce IFNG synthesis in Th1 cells and NK cells. Notably, IL-18 is involved in transduction of inflammation downstream of pyroptosis, where its mature form is released into the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. At the plasma membrane, IL-18 forms a ternary complex with the ligand-binding receptor subunit IL18R1 and signaling receptor subunit IL18RAP, initiating intracellular signaling. Additionally, IL-18 interacts with the cargo receptor TMED10, facilitating its translocation from the cytoplasm into the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and subsequent secretion.

The specific activity is > 1×10⁵ U/mg by ELISA.

Human

HEK293

C-6*His

Q14116 (Y37-D193)

3606  [NCBI]

YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED

Approximately 17 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in injection water.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.