gucD Protein, Bacillus subtilis (His, StrepⅡ)
The gucD protein is an important enzyme that catalyzes the irreversible ammonia removal of L-kynurenine to generate kynurenic acid (KA). This process is part of the tryptophan catabolic pathway and produces KA, known for its broad-spectrum antagonistic activity at excitatory amino acid receptors. gucD Protein, Bacillus subtilis (His, Strep) is the recombinant gucD protein, expressed by E. coli , with N-Strep, N-6*His labeled tag.
- Species: Others
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The gucD protein is an important enzyme that catalyzes the irreversible ammonia removal of L-kynurenine to generate kynurenic acid (KA). This process is part of the tryptophan catabolic pathway and produces KA, known for its broad-spectrum antagonistic activity at excitatory amino acid receptors. gucD Protein, Bacillus subtilis (His, Strep) is the recombinant gucD protein, expressed by E. coli , with N-Strep, N-6*His labeled tag.
Background
KYAT1 protein orchestrates the irreversible transamination of the L-tryptophan metabolite L-kynurenine, culminating in the formation of kynurenic acid (KA). This process holds significance within the tryptophan catabolic pathway and yields KA, known for its broad-spectrum antagonistic activity against the three ionotropic excitatory amino acid receptors, among others. Beyond its role in tryptophan metabolism, KYAT1 is involved in the transformation of cysteine conjugates from specific halogenated alkenes and alkanes into reactive metabolites. Furthermore, KYAT1 catalyzes the beta-elimination of S-conjugates and Se-conjugates of L-(seleno)cysteine, leading to the cleavage of the C-S or C-Se bond. The diverse enzymatic activities of KYAT1 underscore its involvement in key metabolic pathways and xenobiotic processing.
Technical Parameters
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Species Others
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Source E. coli
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Tag N-StrepⅡ;N-6*His
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Accession
P42236 (S2-P488)
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Gene ID938406
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Molecular Construction
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N-term
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StrepⅡ-6*His
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gucD (S2-P488)
Accession # P42236 -
C-term
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Protein Length
Full Length
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Synonyms
BSU02470; ycbD; gucD; 2,5-dioxovalerate dehydrogenase; alphaKGSA dehydrogenase; EC:1.2.1.26; Alpha-ketoglutaric semialdehyde dehydrogenase
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AA Sequence
SVITEQNTYLNFINGEWVKSQSGDMVKVENPADVNDIVGYVQNSTAEDVERAVTAANEAKTAWRKLTGAERGQYLYKTADIMEQRLEEIAACATREMGKTLPEAKGETARGIAILRYYAGEGMRKTGDVIPSTDKDALMFTTRVPLGVVGVISPWNFPVAIPIWKMAPALVYGNTVVIKPATETAVTCAKIIACFEEAGLPAGVINLVTGPGSVVGQGLAEHDGVNAVTFTGSNQVGKIIGQAALARGAKYQLEMGGKNPVIVADDADLEAAAEAVITGAFRSTGQKCTATSRVIVQSGIYERFKEKLLQRTKDITIGDSLKEDVWMGPIASKNQLDNCLSYIEKGKQEGASLLIGGEKLENGKYQNGYYVQPAIFDNVTSEMTIAQEEIFGPVIALIKVDSIEEALNIANDVKFGLSASIFTENIGRMLSFIDEIDAGLVRINAESAGVELQAPFGGMKQSSSHSREQGEAAKDFFTAIKTVFVKP
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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