HAI-1 Protein, Human (Biotinylated, HEK293, His-Avi)
HAI-1 Protein serves as an inhibitor of HGFAC, suppressing serine protease activity of ST14/matriptase in vitro and inhibiting TMPRSS13 through the BPTI/Kunitz inhibitor 1 domain. It interacts with HGFAC and TMPRSS13, with the latter interaction promoting TMPRSS13 phosphorylation and cell membrane localization. HAI-1, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived HAI-1 protein, expressed by HEK293, with N-Avi labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
HAI-1 Protein serves as an inhibitor of HGFAC, suppressing serine protease activity of ST14/matriptase in vitro and inhibiting TMPRSS13 through the BPTI/Kunitz inhibitor 1 domain. It interacts with HGFAC and TMPRSS13, with the latter interaction promoting TMPRSS13 phosphorylation and cell membrane localization. HAI-1, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived HAI-1 protein, expressed by HEK293, with N-Avi labeled tag.
Background
The HAI-1 Protein functions as an inhibitor of HGFAC and exhibits inhibitory effects on the serine protease activity of ST14/matriptase in vitro. Through its BPTI/Kunitz inhibitor 1 domain, HAI-1 also impedes the serine protease activity of TMPRSS13. Interacting with HGFAC and TMPRSS13, HAI-1 forms dynamic associations that influence cellular processes. The interaction with HGFAC suggests a regulatory role in the activation of this protease, while the interaction with TMPRSS13 promotes the phosphorylation and cell membrane localization of TMPRSS13. These intricate interactions and inhibitory functions highlight the versatile regulatory role of HAI-1 in modulating serine protease activities, underscoring its significance in cellular homeostasis and proteolytic pathways.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-His
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Accession
O43278-2 (P37-E449)
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Gene ID6692
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Molecular Construction
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N-term
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HAI-1 (G36-E449)
Accession # O43278-2 -
His-Avi
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C-term
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Protein Length
Partial
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Conjugation
Biotin
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Synonyms
SPINT1; MANSC2; Serine Peptidase Inhibitor, Kunitz Type 1; HAI; HAI-1; Serine Protease Inhibitor, Kunitz Type 1; Hepatocyte Growth Factor Activator Inhibitor Type 1; HAI1; Kunitz-Type Protease Inhibitor 1
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AA Sequence
PPPAPPGLPAGADCLNSFTAGVPGFVLDTNASVSNGATFLESPTVRRGWDCVRACCTTQNCNLALVELQPDRGEDAIAACFLINCLYEQNFVCKFAPREGFINYLTREVYRSYRQLRTQGFGGSGIPKAWAGIDLKVQPQEPLVLKDVENTDWRLLRGDTDVRVERKDPNQVELWGLKEGTYLFQLTVTSSDHPEDTANVTVTVLSTKQTEDYCLASNKVGRCRGSFPRWYYDPTEQICKSFVYGGCLGNKNNYLREEECILACRGVQGPSMERRHPVCSGTCQPTQFRCSNGCCIDSFLECDDTPNCPDASDEAACEKYTSGFDELQRIHFPSDKGHCVDLPDTGLCKESIPRWYYNPFSEHCARFTYGGCYGNKNNFEEEQQCLESCRGISKKDVFGLRREIPIPSTGSVE
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Predicted Molecular Mass
49.7 kDa
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Molecular Weight
Approximately 53-63 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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