HAI-1 Protein, Mouse (Biotinylated, HEK293, His-Avi)
The HAI-1 protein is a multifaceted inhibitor that potently inhibits HGFAC and protects against serine proteases such as ST14/matriptase and TMPRSS13. Through its BPTI/Kunitz inhibitor 1 domain, HAI-1 cleverly coordinates inhibition, engaging in a delicate molecular dance with HGFAC and TMPRSS13. HAI-1, Mouse (Biotinylated, HEK293, His-Avi) is the recombinant mouse-derived HAI-1 protein, expressed by HEK293, with N-Avi labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The HAI-1 protein is a multifaceted inhibitor that potently inhibits HGFAC and protects against serine proteases such as ST14/matriptase and TMPRSS13. Through its BPTI/Kunitz inhibitor 1 domain, HAI-1 cleverly coordinates inhibition, engaging in a delicate molecular dance with HGFAC and TMPRSS13. HAI-1, Mouse (Biotinylated, HEK293, His-Avi) is the recombinant mouse-derived HAI-1 protein, expressed by HEK293, with N-Avi labeled tag.
Background
HAI-1, a multifaceted protein, stands as a potent inhibitor in diverse cellular contexts. Notably, it acts as a robust inhibitor of HGFAC, exerting its regulatory influence on this pivotal factor. Functioning as a guardian against serine protease activities, HAI-1 demonstrates inhibitory effects on ST14/matriptase and TMPRSS13, skillfully wielding its BPTI/Kunitz inhibitor 1 domain. The intricate molecular ballet involves direct interactions with HGFAC and TMPRSS13, orchestrating a nuanced dance that not only inhibits but also promotes specific cellular processes. The interplay with TMPRSS13 underscores HAI-1's role in orchestrating the phosphorylation and cellular localization of this serine protease. In these multifaceted interactions, HAI-1 emerges as a versatile guardian, finely tuning the delicate balance of protease activities in cellular environments.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-Avi;C-His
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Accession
Q9R097 (E30-E443)
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Gene ID20732
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Molecular Construction
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N-term
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HAI-1 (E30-E443)
Accession # Q9R097 -
His-Avi
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C-term
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Protein Length
Partial
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Conjugation
Biotin
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Synonyms
SPINT1; MANSC2; Serine Peptidase Inhibitor, Kunitz Type 1; HAI; HAI-1; Serine Protease Inhibitor, Kunitz Type 1; Hepatocyte Growth Factor Activator Inhibitor Type 1; HAI1; Kunitz-Type Protease Inhibitor 1
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AA Sequence
ELPSAPAELPGGAACLSRFTSGVPSFVLDTEASVSNGATFLGSPTARRGWDCVRSCCTTQNCNLALVELQPDGGEDAISACFLMNCLYEQNFVCKFAPKEGFINYLTQELYRSYRELRTRGFGGSRIPRIWMGIDLKVQLQKPLVLNEADNTDWHLLQGDSDVRVERKRPEEVELWGLKEGTYLFQLTRTDSDQPEETANLTITVLTAKQTEDYCLASYKVGRCRGSFPRWYYDPKEQICKSFTFGGCLGNKNNYLREEECMLACKDVQGISPKRHHPVCSGSCHATQFRCSNGCCIDGFLECDDTPDCPDGSDEATCEKYTSGFDELQNIHFLSDKGYCAELPDTGFCKENIPRWYYNPFSERCARFTYGGCYGNKNNFEEEQQCLESCRGISKKDVFGLRREGSIPTVGSAE
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Predicted Molecular Mass
50 kDa
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Molecular Weight
Approximately 53-63 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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