HAI-1 Protein, Mouse (HEK293, Fc)

The HAI-1 protein is a multifaceted inhibitor that potently inhibits HGFAC and protects against serine proteases such as ST14/matriptase and TMPRSS13. Through its BPTI/Kunitz inhibitor 1 domain, HAI-1 cleverly coordinates inhibition, engaging in a delicate molecular dance with HGFAC and TMPRSS13. HAI-1 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived HAI-1 protein, expressed by HEK293 , with C-hFc labeled tag.

HAI-1, a multifaceted protein, stands as a potent inhibitor in diverse cellular contexts. Notably, it acts as a robust inhibitor of HGFAC, exerting its regulatory influence on this pivotal factor. Functioning as a guardian against serine protease activities, HAI-1 demonstrates inhibitory effects on ST14/matriptase and TMPRSS13, skillfully wielding its BPTI/Kunitz inhibitor 1 domain. The intricate molecular ballet involves direct interactions with HGFAC and TMPRSS13, orchestrating a nuanced dance that not only inhibits but also promotes specific cellular processes. The interplay with TMPRSS13 underscores HAI-1's role in orchestrating the phosphorylation and cellular localization of this serine protease. In these multifaceted interactions, HAI-1 emerges as a versatile guardian, finely tuning the delicate balance of protease activities in cellular environments.

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 The IC₅₀ value is 0.35 nM.

Mouse

HEK293

C-hFc

Q9R097 (E30-E443)

20732  [NCBI]

Partial

ELPSAPAELPGGAACLSRFTSGVPSFVLDTEASVSNGATFLGSPTARRGWDCVRSCCTTQNCNLALVELQPDGGEDAISACFLMNCLYEQNFVCKFAPKEGFINYLTQELYRSYRELRTRGFGGSRIPRIWMGIDLKVQLQKPLVLNEADNTDWHLLQGDSDVRVERKRPEEVELWGLKEGTYLFQLTRTDSDQPEETANLTITVLTAKQTEDYCLASYKVGRCRGSFPRWYYDPKEQICKSFTFGGCLGNKNNYLREEECMLACKDVQGISPKRHHPVCSGSCHATQFRCSNGCCIDGFLECDDTPDCPDGSDEATCEKYTSGFDELQNIHFLSDKGYCAELPDTGFCKENIPRWYYNPFSERCARFTYGGCYGNKNNFEEEQQCLESCRGISKKDVFGLRREGSIPTVGSAE

Approximately 80-95 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.