HEXA/Hexosaminidase A Protein, Human (sf9, His)

HEXA (hexosaminidase A) proteolytically hydrolyzes N-acetyl-D-hexosamine and/or sulfated residues in glycocomplexes, including oligosaccharides, glycolipids, and mucopolysaccharides. The activity of isoenzyme S towards anionic disulfated glycans is comparable to that of isoenzyme A, while isoenzyme B can effectively hydrolyze neutral oligosaccharides but cannot hydrolyze anionic disulfated substrates. HEXA/Hexosaminidase A Protein, Human (sf9, His) is the recombinant human-derived HEXA/Hexosaminidase A protein, expressed by Sf9 insect cells , with C-His labeled tag.

HEXA (Hexosaminidase A) protein plays a pivotal role in hydrolyzing the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine residues found in various glycoconjugates, including oligosaccharide moieties from proteins, neutral glycolipids, and specific mucopolysaccharides. Isozyme S exhibits comparable activity to isozyme A on anionic bis-sulfated glycans such as chondroitin-6-sulfate trisaccharide (C6S-3), dermatan sulfate pentasaccharide, and the sulfated glycosphingolipid SM2. In contrast, isozyme B efficiently hydrolyzes neutral oligosaccharides but does not act on these anionic bis-sulfated substrates. Importantly, only isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A, showcasing its specificity and central role in this biochemical process.

Measured by its ability to hydrolyze 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MU-Glc-NAc). The specific activity is >1,250 pmoles/min/μg.

Human

Sf9 insect cells

C-His

AAD13932.1 (L23-T529)

3073  [NCBI]

Partial

MTSSRLWFSLLLAAAFAGRATALWPWPQNFQTSDQRYVLYPNNFQFQYDVSSAAQPGCSVLDEAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVSVVTPGCNQLPTLESVENYTLTINDDQCLLLSETVWGALRGLETFSQLVWKSAEGTFFINKTEIEDFPRFPHRGLLLDTSRHYLPLSSILDTLDVMAYNKLNVFHWHLVDDPSFPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEYARLRGIRVLAEFDTPGHTLSWGPGIPGLLTPCYSGSEPSGTFGPVNPSLNNTYEFMSTFFLEVSSVFPDFYLHLGGDEVDFTCWKSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYGKGYVVWQEVFDNKVKIQPDTIIQVWREDIPVNYMKELELVTKAGFRALLSAPWYLNRISYGPDWKDFYIVEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKLTSDLTFAYERLSHFRCELLRRGVQAQPLNVGFCEQEFEQT

Approximately 64 kDa,based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.