HGF Protein, Cynomolgus (HEK293)

HGF Protein, a potent mitogen, stimulates mature hepatocyte growth and exhibits hepatotrophic properties for various tissues. It acts as an activating ligand for the receptor tyrosine kinase MET, promoting dimerization upon binding. Structurally, HGF Protein comprises alpha and beta chains linked by a disulfide bond. Additionally, it interacts with SRPX2, enhancing HGF's mitogenic activity. HGF Protein, Cynomolgus (HEK293) is the recombinant cynomolgus-derived HGF protein, expressed by HEK293 , with tag free. The total length of HGF Protein, Cynomolgus (HEK293) is 728 a.a., with molecular weight of ~89 & 58 & 34 & 32 kDa, respectively.

HGF Protein is a potent mitogen that stimulates the growth of mature parenchymal hepatocyte cells and exhibits hepatotrophic properties. It also acts as a growth factor for various tissues and cell types. HGF Protein functions as an activating ligand for the receptor tyrosine kinase MET, promoting its dimerization upon binding. Structurally, HGF Protein is composed of an alpha chain and a beta chain that are linked together by a disulfide bond. Additionally, HGF Protein interacts with SRPX2, leading to an increased mitogenic activity of HGF.

1.Measured by its ability to inhibit TGFß1 activity on Mv-1-lu mink lung epithelial cells and the ED₅₀ is 1-10 ng/mL.
2. Immobilized HGF Protein, Cynomolgus, Recombinant at 2 μg/mL (100 μL/well) can bind c-MET Protein, Cynomolgus, Rhesus, Recombinant (His Tag), the EC₅₀ is 60-240 ng/mL.

Cynomolgus

HEK293

Tag Free

C9E9X5 (M1-S728)

102133907  [NCBI]

Approximately 89&58&34&32 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.