HLA-A*02:01&B2M&GP100(YLEPGPVTA) Monomer Protein, Human (Biotinylated, HEK293, His-Avi)

1. Immobilized Biotinylated Human GP100 (HLA-A*02:01) , His Tag at 2 μg/mL (100 μl/Well). Dose response curve for Anti-GP100 (HLA-A*02:01) Ab., hFc tag with the EC₅₀ of ≤0.3 μg/mL determined by ELISA.
2. Loaded HLA-A*0201 GP100 Complex Protein, Human (Biotinylated, YLEPGPVTA, HEK293, His-Avi) on NTA biosensor, can bind Tebentafusp (HY-P99339) with an affinity constant of 7.761E-10 M as determined in BLI assay.

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  Loaded HLA-A*0201 GP100 Complex Protein, Human (Biotinylated, YLEPGPVTA, HEK293, His-Avi) on NTA biosensor, can bind Tebentafusp (HY-P99339) with an affinity constant of 7.761E-10 M as determined in BLI assay.

Human

HEK293

C-Avi;C-8*His

A0A140T913 (G25-T305)&P61769 (I21-M119)&YLEPGPVTA

/&567  [NCBI]

Biotin

The single lysine residue in Avitag is biotinylated through an enzymatic reaction.

52-62 kDa

≥ 95%, as determined by Bis-Tris PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.