HLA-A*02:01&B2M&GP100(YLEPGPVTA) Monomer Protein, Human (Biotinylated, HEK293, His-Avi)
Based on 1 Customer Validation
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Verified Bioactivity
1. Immobilized Biotinylated Human GP100 (HLA-A*02:01) , His Tag at 2 μg/mL (100 μl/Well). Dose response curve for Anti-GP100 (HLA-A*02:01) Ab., hFc tag with the EC50 of ≤0.3 μg/mL determined by ELISA.
2. Loaded HLA-A*0201 GP100 Complex Protein, Human (Biotinylated, YLEPGPVTA, HEK293, His-Avi) on NTA biosensor, can bind Tebentafusp (HY-P99339) with an affinity constant of 7.761E-10 M as determined in BLI assay.
MCE Validation Data
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Bioactivity - BLI
Bioactivity - BLI
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-8*His
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Accession
A0A140T913 (G25-T305)&P61769 (I21-M119)&YLEPGPVTA
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Conjugation
Biotin
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Synonyms
HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100; ME20M
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Predicted Molecular Mass
50.4 kDa
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Molecular Weight
Approximately 52-62 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (233 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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