1. Recombinant Proteins
  2. Biotinylated Proteins
  3. HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein, Human (Biotinylated, HEK293, His-Avi)

HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P77689
Handling Instructions Technical Support

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity

Immobilized Anti-HLA-G Antibody, hFc Tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human HLAG&B2M&Peptide (RIIPRHLQL) Monomer, His Tag with the EC50 of 10-50 ng/mL determined by ELISA.

Species

Human

Source

HEK293

Tag

C-Avi;C-8*His

Accession

P17693 (G25-T305)&P61769 (I21-M119)&RIIPRHLQL

Gene ID

3135  [NCBI]&567  [NCBI]

Conjugation

Biotin

Conjugate Method

The single lysine residue in Avitag is biotinylated through an enzymatic reaction.

Synonyms
HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G
Predicted Molecular Mass
50.5 kDa
Molecular Weight

Approximately 51-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Glycosylation
Yes
Purity

≥ 95%, as determined by Bis-Tris PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein, Human (Biotinylated, HEK293, His-Avi)

 

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Product Name:
HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein, Human (Biotinylated, HEK293, His-Avi)
Cat. No.:
HY-P77689
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