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HMGN1 Protein, Human

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Based on 1 publication(s) in Google Scholar

The HMGN1 protein is a key player in the immune response, binding to the Fc region of immunoglobulin alpha and specifically promoting the production of cytokines. HMGN1 is intricately related to the Fc epsilon RI gamma 2 receptor, can induce tyrosine phosphorylation of gamma 2, and actively participates in the complex coordination of immune signaling pathways. HMGN1 Protein, Human is the recombinant human-derived HMGN1 protein, expressed by E. coli , with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for HMGN1 Protein, Human
  • Experimental Validation Results for HMGN1 Protein, Human
  • HMGN1 Protein, Human Product Image
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609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The HMGN1 protein is a key player in the immune response, binding to the Fc region of immunoglobulin alpha and specifically promoting the production of cytokines. HMGN1 is intricately related to the Fc epsilon RI gamma 2 receptor, can induce tyrosine phosphorylation of gamma 2, and actively participates in the complex coordination of immune signaling pathways. HMGN1 Protein, Human is the recombinant human-derived HMGN1 protein, expressed by E. coli , with tag free.

Background

FCAR/CD89, a pivotal player in immune responses, exhibits the capability to bind to the Fc region of immunoglobulins alpha, facilitating various functions, notably cytokine production. This multifaceted protein is intricately associated with the Fc epsilon RI gamma 2 receptor, leading to the induction of tyrosine phosphorylation of gamma 2. Through these molecular interactions, FCAR/CD89 actively participates in the complex orchestration of immune signaling pathways, highlighting its role in mediating crucial aspects of the immune response.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized HMGN1 at 2 μg/mL (100 μL/well) can bind anti- HMGN1 Antibody. The ED50 for this effect is 57.03 ng/mL.

Results
  • Experimental Validation Results for HMGN1 Protein, Human
    Measured by its binding ability in a functional ELISA. Immobilized HMGN1 at 2 μg/mL (100 μL/well) can bind anti-HMGN1 Antibody. The ED50 for this effect is 57.03 ng/mL.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    P05114 (P2-D100)

  • Gene ID
    3150  [NCBI]
  • Molecular Construction
    • N-term
    • HMGN1 (P2-D100)
      Accession # P05114
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    Non-histone chromosomal protein HMG-14; HMGN1; High mobility group nucleosome-binding domain-containing protein 1; HMG14; High Mobility Group Nucleosome Binding Domain 1

  • AA Sequence

    PKRKVSSAEGAAKEEPKRRSARLSAKPPAKVEAKPKKAAAKDKSSDKKVQTKGKRGAKGKQAEVANQETKEDLPAENGETKTEESPASDEAGEKEAKSD

  • Predicted Molecular Mass

    10.5 kDa

  • Molecular Weight

    Approximately 19 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

    • Experimental Validation Results for HMGN1 Protein, Human
      ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 500 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

  • ≥ 95%, as determined by reducing SDS-PAGE.

  • Measured by its binding ability in a functional ELISA. Immobilized HMGN1 at 2 μg/mL (100 μL/well) can bind anti-HMGN1 Antibody. The ED50 for this effect is 57.03 ng/mL.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized HMGN1 at 2 μg/mL (100 μL/well) can bind anti-HMGN1 Antibody. The ED50 for this effect is 57.03 ng/mL.

  • HMGN1 Protein, Human Product Image
    1/16
  • Experimental Validation Results for HMGN1 Protein, Human
  • Experimental Validation Results for HMGN1 Protein, Human
  • HMGN1 Protein, Human Product Image