HMGN1 Protein, Human

The HMGN1 protein is a key player in the immune response, binding to the Fc region of immunoglobulin alpha and specifically promoting the production of cytokines. HMGN1 is intricately related to the Fc epsilon RI gamma 2 receptor, can induce tyrosine phosphorylation of gamma 2, and actively participates in the complex coordination of immune signaling pathways. HMGN1 Protein, Human is the recombinant human-derived HMGN1 protein, expressed by E. coli , with tag free.

FCAR/CD89, a pivotal player in immune responses, exhibits the capability to bind to the Fc region of immunoglobulins alpha, facilitating various functions, notably cytokine production. This multifaceted protein is intricately associated with the Fc epsilon RI gamma 2 receptor, leading to the induction of tyrosine phosphorylation of gamma 2. Through these molecular interactions, FCAR/CD89 actively participates in the complex orchestration of immune signaling pathways, highlighting its role in mediating crucial aspects of the immune response.

Measured by its binding ability in a functional ELISA. Immobilized HMGN1 at 2 μg/mL (100 μL/well) can bind anti- HMGN1 Antibody. The ED₅₀ for this effect is 57.03 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized HMGN1 at 2 μg/mL (100 μL/well) can bind anti-HMGN1 Antibody. The ED₅₀ for this effect is 57.03 ng/mL.

Human

E. coli

Tag Free

P05114 (P2-D100)

3150  [NCBI]

Full Length

PKRKVSSAEGAAKEEPKRRSARLSAKPPAKVEAKPKKAAAKDKSSDKKVQTKGKRGAKGKQAEVANQETKEDLPAENGETKTEESPASDEAGEKEAKSD

Approximately 19 kDa, based on SDS-PAGE under reducing conditions.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 500 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.