HNRNPM Protein, Human (His-SUMO)

The HNRNPM protein is a key pre-mRNA binding protein that exhibits strong affinity for poly(G) and poly(U) RNA homopolymers. It is central to the splicing process, complexly regulates RNA maturation, and serves as a receptor for carcinoembryonic antigen in Kupffer cells. HNRNPM Protein, Human (His-SUMO) is the recombinant human-derived HNRNPM protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of HNRNPM Protein, Human (His-SUMO) is 729 a.a., with molecular weight of ~93.4 kDa.

The HNRNPM protein emerges as a crucial pre-mRNA binding protein in vivo, exhibiting a strong affinity for poly(G) and poly(U) RNA homopolymers in vitro. Functionally implicated in splicing processes, HNRNPM plays a significant role in the intricate regulation of RNA maturation. Beyond its involvement in splicing, it acts as a receptor for carcinoembryonic antigen in Kupffer cells, potentially initiating a cascade of signaling events leading to the tyrosine phosphorylation of proteins and the induction of cytokines such as IL-1 alpha, IL-6, IL-10, and tumor necrosis factor alpha. Notably, HNRNPM is identified as a component of the spliceosome C complex, underscoring its association with fundamental RNA processing machinery. Moreover, the protein interacts with PPIA/CYPA, further expanding its network of molecular interactions and highlighting its multifaceted role in cellular processes.

Human

E. coli

N-6*His;N-SUMO

P52272 (A2-A730)

4670  [NCBI]

AAGVEAAAEVAATEIKMEEESGAPGVPSGNGAPGPKGEGERPAQNEKRKEKNIKRGGNRFEPYANPTKRYRAFITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHARRAMQKVMATTGGMGMGPGGPGMITIPPSILNNPNIPNEIIHALQAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMDERALPKGDFFPPERPQQLPHGLGGIGMGLGPGGQPIDANHLNKGIGMGNIGPAGMGMEGIGFGINKMGGMEGPFGGGMENMGRFGSGMNMGRINEILSNALKRGEIIAKQGGGGGGGSVPGIERMGPGIDRLGGAGMERMGAGLGHGMDRVGSEIERMGLVMDRMGSVERMGSGIERMGPLGLDHMASSIERMGQTMERIGSGVERMGAGMGFGLERMAAPIDRVGQTIERMGSGVERMGPAIERMGLSMERMVPAGMGAGLERMGPVMDRMATGLERMGANNLERMGLERMGANSLERMGLERMGANSLERMGPAMGPALGAGIERMGLAMGGGGGASFDRAIEMERGNFGGSFAGSFGGAGGHAPGVARKACQIFVRNLPFDFTWKMLKDKFNECGHVLYADIKMENGKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRIDRNA

Approximately 93.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.