IDH1 Protein, Human (R132H, His)

The IDH1 protein catalyzes the oxidative decarboxylation of isocitrate to generate 2-oxoglutarate, which is utilized by enzymes such as phytoacyl-CoA dioxygenase. IDH1 Protein, Human (R132H, His) is the recombinant human-derived IDH1 protein, expressed by E. coli, with C-8*His labeled tag.

IDH1 protein is a key enzyme that catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), a vital step in cellular metabolism and a process required by other enzymes such as the phytanoyl-CoA dioxygenase. The enzymatic activity of IDH1 is crucial for the generation of NADPH, a critical cofactor involved in various biosynthesis pathways. Additionally, IDH1 may have a distinct role as a corneal epithelial crystallin, potentially contributing to the maintenance of corneal epithelial transparency. These multifaceted functions highlight the importance of IDH1 in fundamental cellular processes and suggest its involvement in maintaining specific tissue characteristics.

Human

E. coli

C-8*His

O75874 (M1-L414, R132H)

3417

Full Length

MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIIIGHHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNKELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKL

Approximately 40-50 kDa,based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50mM Tris-HCl, 4% Sucrose, 50% glycerol, 0.02% Tween80, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.