IDS/Iduronate 2-sulfatase Protein, Human (HEK293, His)

IDS/Iduronate 2-sulfatase Protein, a lysosomal enzyme, participates in the degradation pathway of dermatan sulfate and heparan sulfate. IDS/Iduronate 2-sulfatase Protein, Human (HEK293, His) is the recombinant human-derived IDS/Iduronate 2-sulfatase protein, expressed by HEK293 , with C-His labeled tag. The total length of IDS/Iduronate 2-sulfatase Protein, Human (HEK293, His) is 525 a.a., with molecular weight (glycosylation form) of ~75-95 kDa.

IDS (Iduronate 2-sulfatase) is a lysosomal enzyme playing a crucial role in the degradation pathway of dermatan sulfate and heparan sulfate. As an essential component of cellular metabolism, IDS facilitates the breakdown of these glycosaminoglycans within lysosomes, contributing to the maintenance of normal cellular function and tissue homeostasis. The enzymatic activity of IDS is vital for the catabolism of dermatan sulfate and heparan sulfate, preventing their accumulation within lysosomal compartments and ensuring proper cellular processes. This underscores the pivotal role of IDS in the intricate network of metabolic pathways involved in the degradation of glycosaminoglycans.

Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS). The specific activity is 6.123 pmoL/min/μg, as measured under the described conditions.

Human

HEK293

C-10*His

P22304-1 (S26-P550)

3423  [NCBI]

SETQANSTTDALNVLLIIVDDLRPSLGCYGDKLVRSPNIDQLASHSLLFQNAFAQQAVCAPSRVSFLTGRRPDTTRLYDFNSYWRVHAGNFSTIPQYFKENGYVTMSVGKVFHPGISSNHTDDSPYSWSFPPYHPSSEKYENTKTCRGPDGELHANLLCPVDVLDVPEGTLPDKQSTEQAIQLLEKMKTSASPFFLAVGYHKPHIPFRYPKEFQKLYPLENITLAPDPEVPDGLPPVAYNPWMDIRQREDVQALNISVPYGPIPVDFQRKIRQSYFASVSYLDTQVGRLLSALDDLQLANSTIIAFTSDHGWALGEHGEWAKYSNFDVATHVPLIFYVPGRTASLPEAGEKLFPYLDPFDSASQLMEPGRQSMDLVELVSLFPTLAGLAGLQVPPRCPVPSFHVELCREGKNLLKHFRFRDLEEDPYLPGNPRELIAYSQYPRPSDIPQWNSDKPSLKDIKIMGYSIRTIDYRYTVWVGFNPDEFLANFSDIHAGELYFVDSDPLQDHNMYNDSQGGDLFQLLMP

Approximately 75-95 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.