IFN-alpha 7/IFNA7 Protein, Human (P.pastoris, His)
Based on 1 Customer Validation
IFN-alpha 7 (IFNA7), belongs to type I interferon family, is produced by macrophages with antiviral activities. IFN-alpha 7/IFNA7 Protein, Human (P.pastoris, His) contains 166 a.a. (C24-D189), produced in P.pastoris yeast cells with a C-terminal His-tag.
- Species: Human
- Source: P. pastoris
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
IFN-alpha 7 (IFNA7), belongs to type I interferon family, is produced by macrophages with antiviral activities[1]. IFN-alpha 7/IFNA7 Protein, Human (P.pastoris, His) contains 166 a.a. (C24-D189), produced in P.pastoris yeast cells with a C-terminal His-tag.
Background
IFN-alpha 7 (IFNA7; IFN-α7), belongs to the alpha/beta interferon (IFN) family, is produced by the macrophages with antiviral activities[1]. Interferon (IFN) is originally identified as a substance ‘interfering’ with viral replication in vitro. IFN-α/β and related molecules are classified as type I IFNs, as for the other two types of type II IFN (IFN-γ) and type III IFNs (IFN-λ), respectively[2].
Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Interferon alpha (IFNa) shows significant biological activity in various cancers, paticularly haematological malignancies such as hairy cell leukaemia and chronic myelogenous leukaemia[3].
IFNα-7 is identified as positive regulators that increases FOXP3 expression and/or decreases CTLA4 expression. Therefore, regulatory T cells (Tregs) as the key cells regulating peripheral autoreactive T lymphocytes, IFNα-7 regulates Treg functional states to protect Treg phenotype stabilization[4].
As for a wildly use of IFN in animal model, the sequence of amino acids in IFNA7 protein of human is very different from mouse (58.73%)
In Vitro
IFN-alpha 7 (1-1.9-1-6.6 M; 24-26 h) results a signifcant reduction of FOXP3 gene expression in regulatory T cells (Tregs) without affecting cell viability[4].
Verified Bioactivity
Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus. The ED50 for this effect is 9.1 pg/mL.
Technical Parameters
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Species Human
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Source P. pastoris
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Tag C-His
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Accession
P01567/NP_066401.2 (C24-D189)
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Gene ID3444 [NCBI]
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Molecular Construction
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N-term
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IFNA7 (C24-D189)
Accession # P01567/NP_066401.2 -
His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
IFNA7; Interferon Alpha-J; Interferon Alpha 7; IFN-Alpha-J1; IFN-AlphaJ; LeIF J; IFNA-J; Interferon 1AA5; Interferon Alpha-J1; IFN-Alpha 7; Interferon Alpha-7; IFN-Alpha-7
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AA Sequence
CDLPQTHSLRNRRALILLAQMGRISPFSCLKDRHEFRFPEEEFDGHQFQKTQAISVLHEMIQQTFNLFSTEDSSAAWEQSLLEKFSTELYQQLNDLEACVIQEVGVEETPLMNEDFILAVRKYFQRITLYLMEKKYSPCAWEVVRAEIMRSFSFSTNLKKGLRRKD
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Predicted Molecular Mass
21 kDa
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Molecular Weight
Approximately 21 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (264 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Kumagai Y, et al. Alveolar macrophages are the primary interferon-alpha producer in pulmonary infection with RNA viruses. Immunity. 2007 Aug;27(2):240-52. [Content Brief]
[2]. Zhang SY, et al. Inborn errors of interferon (IFN)-mediated immunity in humans: insights into the respective roles of IFN-alpha/beta, IFN-gamma, and IFN-lambda in host defense. Immunol Rev. 2008 Dec;226:29-40. [Content Brief]
[3]. Raj NB, et al. Identification of a novel virus-responsive sequence in the promoter of murine interferon-alpha genes. J Biol Chem. 1991 Jun 15;266(17):11360-5. [Content Brief]
[4]. Ding M, et al. Secretome screening reveals immunomodulating functions of IFNα-7, PAP and GDF-7 on regulatory T-cells. Sci Rep. 2021 Aug 18;11(1):16767. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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