IGFBP-3 Protein, Human (HEK293, Fc)

IGFBP-3 protein is a part of IGFBP that significantly prolongs IGF half-life and modulates growth effects in cell culture. It inhibits or stimulates IGF-induced cellular responses by changing the kinetics of interaction with cell surface receptors. IGFBP-3 Protein, Human (HEK293, Fc) is the recombinant human-derived IGFBP-3 protein, expressed by HEK293, with C-hFc labeled tag.

IGFBP-3 Protein, a member of the insulin-like growth factor-binding proteins (IGFBPs), plays a crucial role in extending the half-life of insulin-like growth factors (IGFs) and modulating their growth-promoting effects on cell culture. With the capacity to either inhibit or stimulate IGF-induced cellular responses, IGFBP-3 exerts its regulatory influence by altering the interaction dynamics between IGFs and their respective cell surface receptors. Additionally, IGFBP-3 exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Furthermore, IGFBP-3 inhibits the positive effect of humanin on insulin sensitivity and promotes testicular germ cell apoptosis. Interactions with XLKD1 and the formation of a ternary complex with IGF1 or IGF2 and a glycoprotein (ALS) further underscore the diverse roles of IGFBP-3. The intricate network of interactions, including those with humanin and TMEM219, highlights the multifaceted nature of IGFBP-3 in modulating cellular processes and signaling pathways associated with growth and apoptosis.

Human

HEK293

C-hFc

P17936-1 (G28-K291)

3486

Full Length of Mature Peptide

GASSAGLGPVVRCEPCDARALAQCAPPPAVCAELVREPGCGCCLTCALSEGQPCGIYTERCGSGLRCQPSPDEARPLQALLDGRGLCVNASAVSRLRAYLLPAPPAPGNASESEEDRSAGSVESPSVSSTHRVSDPKFHPLHSKIIIIKKGHAKDSQRYKVDYESQSTDTQNFSSESKRETEYGPCRREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGRKRGFCWCVDKYGQPLPGYTTKGKEDVHCYSMQSK

Approximately 65-80 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.