IGFBP-4 Protein, Cynomolgus (HEK293, His)
Based on 1 Customer Validation
IGFBP-4 protein lacks essential conserved residue(s) for propagating feature annotation. IGFBP-4 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived IGFBP-4 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Cynomolgus
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
IGFBP-4 protein lacks essential conserved residue(s) for propagating feature annotation. IGFBP-4 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived IGFBP-4 protein, expressed by HEK293 , with C-His labeled tag.
Background
IGFBP-4 Protein lacks conserved residue(s) required for the propagation of feature annotation.
Verified Bioactivity
Measured by its ability to inhibit the biological activity of IGFII on MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 0.04-0.2 μg/mL in the presence of 14 ng/mL human IGFII.
Technical Parameters
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Species Cynomolgus
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Source HEK293
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Tag C-His
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Accession
XP_005584157.1 (D22-E258)
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Molecular Construction
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N-term
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IGFBP-4 (D22-E256)
Accession # A0A2K5VE67 -
His
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C-term
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Protein Length
Partial
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Synonyms
IGFBP4; IGF-Binding Protein 4; Insulin Like Growth Factor Binding Protein 4; HT29-IGFBP; IGFBP-4; BP-4; IBP4; IBP-4; Insulin-Like Growth Factor-Binding Protein 4; Insulin-Like Growth Factor Binding Protein 4
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AA Sequence
DEAIHCPPCSEEKLARCRPPVGCEELVREPGCGCCATCALGLGMPCGVYTPRCGSGLRCYPPRGVEKPLHTLMHGQGVCMELAEIEAIQESLQPSDKDEGDHPNNSFSPCSAHDRRCLQKHFAKIRDRSTSGGKMKVNGAPREDARPVPQGSCQSELHRALERLAASQSRTHEDLYIIPIPNCDRNGNFHPKQCHPALDGQRGKCWCVDRKTGVKLPGGLEPKGELDCHQLADSFRE
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Predicted Molecular Mass
27.4 kDa
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Molecular Weight
Approximately 33-37 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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